FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC49440.12359999999999999No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG40580.10145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAC60000.011.017884
TCCGAG21750.010.46600245
GGACGT26500.010.3507656
GTCCTA65150.010.0333471
AGGACG29750.09.8255835
GACGTG26650.09.698927
TAGACG12600.08.7443494
TCTCCG26150.08.53261743
CTCCGA26900.08.37850444
TCCTCG24500.08.09534544
ACTGCG10000.08.0918348
TGTAGG64750.08.0565692
GTGTAG50650.07.7879091
CTCTTA238550.07.75679845
TAAGAC44400.07.7483714
GTATAG39850.07.69259451
GTCGTA9600.07.5134781
TACGGT5405.895936E-87.49262614
CCTCGC22400.07.445646345
TCTCTT326400.07.360812745
Produced by FastQC (version 0.11.3)