FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L001_R1_015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L001_R1_015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979926
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGA2505.4542397E-912.58627623
GGACGT20850.010.3501656
TAGGAC48100.010.0965024
AGGACG23750.09.7507025
CGTCTA9950.09.7419621
CCGACG3302.5188183E-79.53505822
GACGTG22300.09.4742937
GTCCTA49200.09.20941451
GTCGTA6500.09.0169781
GGTCGG5753.6379788E-128.99125411
TCCGAG15950.08.33690245
CTCTTA159350.08.0760245
GTATAG28800.07.90551041
TGTAGG46750.07.83913762
TCTCCG17250.07.70861343
TCTCTT216900.07.647713745
GCTCGA6402.388333E-97.37563711
CCGGTC5802.4802375E-87.3635269
TGCGAC4602.7161823E-67.32933420
CTATAC30400.07.32190134
Produced by FastQC (version 0.11.3)