FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC58300.14575No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG45780.11445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT28400.011.31932456
AGGACG32200.011.172175
TAGGAC63950.010.8640384
GTCCTA66400.010.3626351
GACGTG29550.010.1180537
TCCGAG19850.09.42753245
CGACGA3403.7671098E-68.59542323
CTATAC39150.08.3274054
TGTAGG65700.08.3159972
CGTCTA12600.08.2092621
GTATAG38100.07.96756551
TCTCTT325100.07.86459845
CTCTTA231150.07.656956745
TCTCCG24450.07.653845344
GGACCG6901.7007551E-107.49344546
CTACAC50500.07.4798234
CCTACA70800.07.4629073
CTGTAG70250.07.4260611
GTTCGC4257.539285E-67.40528739
ACGTGG23150.07.38015378
Produced by FastQC (version 0.11.3)