FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC53140.13285No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG44490.11122499999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAC62550.011.5034834
GGACGT28050.09.6155886
AGGACG31450.09.5805585
GTCCTA65600.09.3586751
CCGACG3852.1549567E-89.34085722
TCTCCG24150.09.24804644
TGCGAC5601.8189894E-129.23162510
GACGTG29550.08.89937
TGTAGG66500.08.5209022
ACGCCG4054.4529452E-78.3246076
GTAGGA63750.08.3241223
GCGAGA27400.08.2851120
CGAACG2459.748311E-48.2764130
CGACGA4351.3938734E-78.26719623
TGGCGA40900.08.24329618
GCGACC7400.08.20106711
TCCGAG20750.08.15408145
CGTCTA13000.08.1303651
CTCCGA27150.07.976884445
GTATAG39500.07.97051671
Produced by FastQC (version 0.11.3)