FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC46500.11624999999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGA3402.642082E-1011.23426523
CCGACG3902.3010216E-1010.37060922
GTCCTA67150.09.8075191
CGAACG3454.2458396E-89.79549830
GGACGT29300.09.4324176
TAGGAC65250.09.2664794
GACGTG28400.09.2566337
AGGACG31850.09.1036985
TCCGAG20600.08.22718745
CTCTTA240100.07.915187445
TCTCCG24950.07.879634444
GTGTAG49500.07.81017541
CCTACA71750.07.76913643
TCTCGC18000.07.65798843
TCTCTT326250.07.625953745
ACTGCG10100.07.56394678
GCGACC7307.230483E-117.387468311
TCCTAC64700.07.36503032
CGTACG5802.4988367E-87.360656734
TGCGAC5508.057532E-87.35388810
Produced by FastQC (version 0.11.3)