FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC47110.11777499999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT28750.010.554636
CGACGA3453.9531187E-910.42263223
GACGTG26400.010.3873087
TAGGAC63700.010.2706624
AGGACG31600.010.1029025
TCCGAG20500.09.9081745
GTCCTA65300.09.7416211
CCGACG4208.442839E-99.0968822
TACGCG3801.7757657E-78.8746775
GTATAG39150.08.4974371
CTACAC50400.08.4309434
TCTCCG24000.08.27515743
TGTAGG68150.07.7197952
GTGTAG51800.07.68071271
CTCCGA26450.07.593974644
TCTCGC20100.07.5228743
CTATAC41900.07.40474224
CTCTTA215650.07.294375445
TACACT54300.07.24574575
CTGTAG72050.07.1130991
Produced by FastQC (version 0.11.3)