FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC57220.14304999999999998No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG45440.1136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT30300.010.9127066
GACGTG30800.010.7355527
AGGACG32300.010.5158195
GTCCTA68550.010.5022361
TAGGAC61750.010.1268994
TACGCG3659.345513E-99.8604295
CGTCTA11900.09.0747271
CGCGTA4054.6344212E-88.8863367
ACGCGT4351.4709258E-88.7905796
TCCGAG20550.08.76867545
TGTAGG65350.08.4335582
CGACGA3201.7280247E-58.43296723
TCTCCG26350.08.20607744
ACGTGG25250.08.19177158
GTATAG38600.07.98495671
CTACAC52050.07.90858944
TCGTCG2300.00546205287.833639629
CTCCGA27050.07.8273845
GTCGTC9300.07.7364229
TCCTAC64500.07.67279052
Produced by FastQC (version 0.11.3)