FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC52530.131325No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG40930.10232500000000001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT27800.010.8410156
TAGACG11850.010.6290864
GTCCTA63550.010.4887661
GACGTG27850.010.4177627
AGGACG31000.010.3752985
TAGGAC64600.010.027354
TCCGAG21750.09.53075145
TGTAGG60400.08.6020362
CGTTCG3059.5253745E-58.109524
CTATAC39950.08.050924
GCGCAA5955.802576E-107.93424911
TCTCTT334300.07.82518145
TACGGT4901.00490524E-77.79960614
GTGTAG49650.07.6650621
CTCCGA27400.07.56546944
GTAGGA65150.07.4915483
CTCTTA239100.07.46352845
GTATAG38200.07.42771771
TCTCCG27400.07.400723543
TTCGTC20400.07.400195644
Produced by FastQC (version 0.11.3)