FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC48220.12054999999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT26650.011.1461326
GACGTG25800.010.8958417
TAGGAC59800.010.6880214
AGGACG31500.010.2880265
GTCCTA64250.09.7166771
TCCGAG20700.08.27577745
GTATAG41600.08.2349581
CTCTTA242650.08.13746945
TGTAGG63800.07.86618762
CTATAC41000.07.6297634
TCTCTT331650.07.550915745
CGTCTA12600.07.51259331
TAGACG13350.07.4173974
TACGGT5802.4781457E-87.364142414
ACGTGG21750.07.3387638
GTAGGA63950.07.3198623
TCCTAC59450.07.3060992
TTATAC148600.07.22027445
CCGACG4053.3377226E-57.21580814
ACTGCG10350.07.1679898
Produced by FastQC (version 0.11.3)