FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC56160.1404No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG43910.109775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT27850.011.472696
GACGTG27400.010.8380087
TAGGAC62750.010.6876114
AGGACG32100.010.3761155
GTCCTA69450.09.7895981
TCCGAG18400.09.66474845
CGAACG3059.39503E-79.59095530
TGCGAC5804.092726E-128.92058610
ACTGCG10650.08.871428
CGTCTA12350.08.3853471
TGTAGG64050.08.2570872
GTAGGA63300.07.64386223
TCTCCG21050.07.59254844
CGTTCG3657.919488E-57.395743411
ACCGTC6107.589733E-97.3755378
TCTCTT299300.07.28031145
GTGTAG49650.07.2548921
CGTCGG2800.00325811777.23273930
TCTCGC21000.07.18167843
CTCCGA23200.07.1800144
Produced by FastQC (version 0.11.3)