FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC57280.1432No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG45560.1139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT28050.011.1492566
AGGACG30800.010.8148775
TAGGAC62600.010.7140214
GACGTG28650.010.44405657
GTCCTA68800.010.4361421
CGTCGT3252.1097744E-68.99916528
CCGACG3353.0931105E-68.73086122
TCCGAG20600.08.5209745
CGACGA3454.482803E-68.47747423
TCGTCG2408.19786E-48.43671729
CGAACG2956.610458E-58.38905130
TGTAGG62900.08.1582072
CCTACA74200.08.0987483
TCTCTT321000.08.03417445
GTAGGA64050.08.0117293
TCTCCG24550.07.97497644
TAGACG13300.07.95347174
TCCTAC64950.07.9007122
ACGTGG23850.07.82946168
CTCTTA232400.07.78541245
Produced by FastQC (version 0.11.3)