FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC50900.12725No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG42480.1062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAC60750.010.7800484
GGACGT27250.010.402876
GTCCTA64700.010.1910991
GACGTG27850.010.1787497
AGGACG30250.010.0434375
TACGCG3801.6704157E-68.291225
CGCGTA4258.739039E-77.9405657
TCCGAG21000.07.930638345
CGTCTA12950.07.8198861
TAGACG13300.07.7835944
TCCTCG25250.07.665364344
CTATAC38500.07.6574574
TCTCTT328600.07.609256745
CTCTTA235700.07.552881245
GTATAG40550.07.5475461
ACGTGG23750.07.3889058
CTGTAG68000.07.3799541
TCTCCG22900.07.36971843
TGTAGG64400.07.3383192
CCTACA68900.07.3163073
Produced by FastQC (version 0.11.3)