FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC45670.114175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAC63050.011.5623264
GGACGT28150.010.2303316
GTCCTA65450.010.1756441
TCCGAG20450.09.68342445
GACGTG27900.09.5153577
AGGACG32400.09.5139495
TACGGT4851.0177246E-98.81393914
TCTCCG23150.08.26180643
CTCTTA220950.07.984756545
CTCCGA24550.07.9752744
TCTCTT306900.07.83827345
CCTACA64400.07.82613753
TGTAGG63150.07.80299762
CGTCTA12700.07.79522751
GTATAG40700.07.7947931
TGGCGA37700.07.758379518
TAGACG13100.07.7290574
GTGTAG48000.07.68745331
CGAACG3251.8685145E-47.61543330
GTAGGA60900.07.50004773
Produced by FastQC (version 0.11.3)