FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC55840.1396No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG47460.11865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT29000.012.48613556
GACGTG29250.011.7641437
AGGACG32400.011.38410955
TAGGAC63450.010.9174014
TCCGAG21000.010.0712345
GTCCTA65750.09.7836981
GTATAG39100.09.0889381
TGTAGG67400.09.0769882
ACGTGG23000.08.8005428
CGAACG3101.1817103E-58.7111430
CGACGA3252.0684884E-58.30410115
GTGTAG52150.08.1946811
GTAGGA65250.08.0311113
TACGGT5355.907623E-97.987200714
TCTCCG25500.07.8657743
GATCGA3201.5906164E-47.7310327
CGTGGA26000.07.6985949
CTCTTA242800.07.691363345
CGTACG4104.6522114E-67.679606410
CTCCGA25700.07.628410344
Produced by FastQC (version 0.11.3)