FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC49680.1242No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG40380.10095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCG3950.011.9551445
CGCGTA4351.3187673E-1110.3387487
GTCCTA61200.010.288711
GGACGT29050.010.217876
GACGTG28300.010.1706977
AGGACG31250.010.146165
CGACGA2904.9757364E-710.08027823
TAGGAC66100.010.0018074
CGTCTA13150.08.8926651
CCGACG3353.113972E-68.72621122
ACGCGT4951.4815669E-98.6313956
CGAACG3403.7018526E-68.60735230
TCCGAG20300.08.32050745
GTATAG40150.08.121511
CTATAC38300.08.0436734
GATCGG4053.955947E-67.77369171
TGTAGG66700.07.7545452
TCTCTT322100.07.691065345
TCGTCG2650.0019630417.655870439
CTCTTA234950.07.610787445
Produced by FastQC (version 0.11.3)