FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC44640.1116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGA3005.6311364E-911.2427623
TAGGAC64200.010.8231464
GGACGT27850.010.8194266
AGGACG32300.010.1643695
GACGTG28350.010.0728787
GTCCTA65800.09.9448261
CCGACG3801.7510501E-89.467586522
TGTAGG64600.08.6327512
TCCGAG21300.08.14873545
CTACAC50500.07.83702664
TACGCG3751.2808918E-57.7954675
CTCTTA235350.07.62391445
GCGTAG7103.8653525E-117.6012141
TCTCTT322650.07.59411245
ACGTGG22850.07.5771778
CGTCTA11900.07.55862951
CTGTAG73250.07.5212241
CGCGTA3302.2143351E-47.49517257
CTCCGA27800.07.459713545
GTGTAG49600.07.4351931
Produced by FastQC (version 0.11.3)