FastQCFastQC Report
Wed 27 May 2015
15253_CGTACT_L002_R1_015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15253_CGTACT_L002_R1_015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212819
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC22370.10109276899737393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGT14250.012.149126
AGGACG15900.011.7368075
TAGGAC32500.011.5531944
GACGTG16550.010.5946617
GTCCTA38250.010.2286011
GTTCGC2305.17781E-59.7737829
TCGCGT1650.00446690259.53684125
CGACGA1700.00556181329.25634623
CGTCGT2254.5283613E-49.00693928
CGTTCG2150.00320636888.36453924
TGTAGG36900.08.347632
TCCGAG10300.08.10876145
CCTCGC11200.08.061845
TCTCCG11500.08.04777944
GTAGGA35250.07.97296523
CTGTAG40600.07.7535471
GTATAG21850.07.7180621
CCGGTA3555.7925325E-57.59877135
ACGTGG12150.07.5857428
GTATAA26800.07.5510291
Produced by FastQC (version 0.11.3)