2 from django.db import models
3 from django.contrib.auth.models import User
4 from htsworkflow.frontend import settings
5 from htsworkflow.frontend.reports.libinfopar import *
7 # Create your models here.
9 class Antibody(models.Model):
10 antigene = models.CharField(max_length=500, db_index=True)
13 # alter table fctracker_antibody add column "nickname" varchar(20) NULL;
14 nickname = models.CharField(
19 verbose_name = 'Short Name'
21 catalog = models.CharField(max_length=50, unique=True, db_index=True)
22 antibodies = models.CharField(max_length=500, db_index=True)
23 source = models.CharField(max_length=500, blank=True, db_index=True)
24 biology = models.TextField(blank=True)
25 notes = models.TextField(blank=True)
26 def __unicode__(self):
27 return u'%s - %s (%s)' % (self.antigene, self.antibodies, self.catalog)
29 verbose_name_plural = "antibodies"
30 ordering = ["antigene"]
32 class Cellline(models.Model):
33 cellline_name = models.CharField(max_length=100, unique=True, db_index=True)
34 nickname = models.CharField(max_length=20,
38 verbose_name = 'Short Name')
39 notes = models.TextField(blank=True)
40 def __unicode__(self):
41 return unicode(self.cellline_name)
44 ordering = ["cellline_name"]
46 class Condition(models.Model):
47 condition_name = models.CharField(
48 max_length=2000, unique=True, db_index=True)
49 nickname = models.CharField(max_length=20,
53 verbose_name = 'Short Name')
54 notes = models.TextField(blank=True)
56 def __unicode__(self):
57 return unicode(self.condition_name)
60 ordering = ["condition_name"]
62 class Tag(models.Model):
63 tag_name = models.CharField(max_length=100, db_index=True,blank=False,null=False)
65 #('Antibody','Antibody'),
66 #('Cellline', 'Cellline'),
67 #('Condition', 'Condition'),
68 ('Library', 'Library'),
71 context = models.CharField(max_length=50,
72 choices=TAG_CONTEXT, default='Library')
74 def __unicode__(self):
75 return u'%s' % (self.tag_name)
78 ordering = ["context","tag_name"]
80 class Species(models.Model):
81 scientific_name = models.CharField(max_length=256,
85 common_name = models.CharField(max_length=256, blank=True)
86 #use_genome_build = models.CharField(max_length=100, blank=False, null=False)
88 def __unicode__(self):
89 return u'%s (%s)' % (self.scientific_name, self.common_name)
92 verbose_name_plural = "species"
93 ordering = ["scientific_name"]
95 class Affiliation(models.Model):
96 name = models.CharField(max_length=256, db_index=True, verbose_name='Group Name')
97 contact = models.CharField(max_length=256, null=True, blank=True,verbose_name='Contact Name')
98 email = models.EmailField(null=True,blank=True)
100 def __unicode__(self):
101 str = unicode(self.name)
102 if self.contact is not None and len(self.contact) > 0:
103 str += u' ('+self.contact+u')'
107 ordering = ["name","contact"]
108 unique_together = (("name", "contact"),)
110 class Library(models.Model):
111 id = models.AutoField(primary_key=True)
112 library_id = models.CharField(max_length=30, db_index=True)
113 library_name = models.CharField(max_length=100, unique=True)
114 library_species = models.ForeignKey(Species)
115 cell_line = models.ForeignKey(Cellline)
116 condition = models.ForeignKey(Condition)
117 antibody = models.ForeignKey(Antibody,blank=True,null=True)
118 # New field Aug/25/08. SQL: alter table fctracker_library add column "lib_affiliation" varchar(256) NULL;
119 affiliations = models.ManyToManyField(Affiliation,related_name='library_affiliations',null=True)
120 # new field Nov/14/08
121 tags = models.ManyToManyField(Tag,related_name='library_tags',blank=True,null=True)
122 # New field Aug/19/08
123 # SQL to add column: alter table fctracker_library add column "replicate" smallint unsigned NULL;
124 REPLICATE_NUM = ((1,1),(2,2),(3,3),(4,4))
125 replicate = models.PositiveSmallIntegerField(choices=REPLICATE_NUM,default=1)
128 ('INPUT_RXLCh','INPUT_RXLCh'),
129 ('ChIP-seq', 'ChIP-seq'),
130 ('Sheared', 'Sheared'),
131 ('RNA-seq', 'RNA-seq'),
132 ('Methyl-seq', 'Methyl-seq'),
133 ('DIP-seq', 'DIP-seq'),
135 experiment_type = models.CharField(max_length=50, choices=EXPERIMENT_TYPES,
138 creation_date = models.DateField(blank=True, null=True)
139 made_for = models.CharField(max_length=50, blank=True,
140 verbose_name='ChIP/DNA/RNA Made By')
141 made_by = models.CharField(max_length=50, blank=True, default="Lorian")
143 PROTOCOL_END_POINTS = (
145 ('Sample', 'Raw sample'),
146 ('Progress', 'In progress'),
147 ('1A', 'Ligation, then gel'),
148 ('PCR', 'Ligation, then PCR'),
149 ('1Ab', 'Ligation, PCR, then gel'),
150 ('1Aa', 'Ligation, gel, then PCR'),
151 ('2A', 'Ligation, PCR, gel, PCR'),
152 ('Done', 'Completed'),
154 stopping_point = models.CharField(max_length=25, choices=PROTOCOL_END_POINTS, default='Done')
155 amplified_from_sample = models.ForeignKey('self', blank=True, null=True)
157 undiluted_concentration = models.DecimalField("Concentration",
158 max_digits=5, decimal_places=2, default=0, blank=True, null=True,
159 help_text="Undiluted concentration (ng/ul)")
160 successful_pM = models.DecimalField(max_digits=5, decimal_places=2, blank=True, null=True)
161 ten_nM_dilution = models.BooleanField()
162 avg_lib_size = models.IntegerField(default=225, blank=True, null=True)
163 notes = models.TextField(blank=True)
165 def __unicode__(self):
166 return u'#%s: %s' % (self.library_id, self.library_name)
169 verbose_name_plural = "libraries"
170 ordering = ["-creation_date"] #["-library_id"]
172 def antibody_name(self):
173 str ='<a target=_self href="/admin/samples/antibody/'+self.antibody.id.__str__()+'/" title="'+self.antibody.__str__()+'">'+self.antibody.nickname+'</a>'
175 antibody_name.allow_tags = True
178 return self.library_species.common_name
180 def affiliation(self):
181 affs = self.affiliations.all().order_by('name')
185 ar.append(t.__unicode__())
186 return '%s' % (", ".join(ar))
189 affs = self.tags.all().order_by('tag_name')
192 ar.append(t.__unicode__())
193 return u'%s' % ( ", ".join(ar))
196 str ='<a target=_self href="/admin/experiments/datarun/?q='+self.library_id+'" title="Check All Data Runs for This Specific Library ..." ">Data Run</a>'
198 DataRun.allow_tags = True
200 def aligned_m_reads(self):
201 return getLibReads(self.library_id)
203 def aligned_reads(self):
204 res = getLibReads(self.library_id)
208 return u'<div style="border:solid red 2px">'+res[2]+'</div>'
210 rc = "%1.2f" % (res[1]/1000000.0)
211 # Color Scheme: green is more than 10M, blue is more than 5M, orange is more than 3M and red is less. For RNAseq, all those thresholds should be doubled
213 bgcolor = '#ff3300' # Red
214 rc_thr = [10000000,5000000,3000000]
215 if self.experiment_type == 'RNA-seq':
216 rc_thr = [20000000,10000000,6000000]
218 if res[1] > rc_thr[0]:
219 bgcolor = '#66ff66' # Green
221 if res[1] > rc_thr[1]:
222 bgcolor ='#00ccff' # Blue
224 if res[1] > rc_thr[2]:
225 bgcolor ='#ffcc33' # Orange
226 tstr = '<div style="background-color:'+bgcolor+';color:black">'
227 tstr += res[0].__unicode__()+' Lanes, '+rc+' M Reads'
229 else: tstr = 'not processed yet'
231 aligned_reads.allow_tags = True
235 summary_url = urlparse.urljoin(SITE_ROOT, 'library/%s' % (self.library_id))
236 return '<a href="%s">S</a>' % (summary_url,)
237 public.allow_tags = True