--- /dev/null
+import shutil
+import sqlite3
+import sys
+
+def main(cmdline=None):
+ if len(cmdline) == 1:
+ dest='/tmp/fctracker.db'
+ else:
+ dest = cmdline[1]
+ shutil.copy(cmdline[0], dest)
+ conn = sqlite3.connect(dest)
+ c = conn.cursor()
+ c.execute('drop table fctracker_elandresult');
+ c.execute('''CREATE TABLE "experiments_flowcell" (
+ "id" integer NOT NULL PRIMARY KEY,
+ "flowcell_id" varchar(20) NOT NULL UNIQUE,
+ "run_date" datetime NOT NULL,
+ "advanced_run" bool NOT NULL,
+ "paired_end" bool NOT NULL,
+ "read_length" integer NOT NULL,
+ "lane_1_library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "lane_2_library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "lane_3_library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "lane_4_library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "lane_5_library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "lane_6_library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "lane_7_library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "lane_8_library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "lane_1_pM" decimal NOT NULL,
+ "lane_2_pM" decimal NOT NULL,
+ "lane_3_pM" decimal NOT NULL,
+ "lane_4_pM" decimal NOT NULL,
+ "lane_5_pM" decimal NOT NULL,
+ "lane_6_pM" decimal NOT NULL,
+ "lane_7_pM" decimal NOT NULL,
+ "lane_8_pM" decimal NOT NULL,
+ "lane_1_cluster_estimate" integer NULL,
+ "lane_2_cluster_estimate" integer NULL,
+ "lane_3_cluster_estimate" integer NULL,
+ "lane_4_cluster_estimate" integer NULL,
+ "lane_5_cluster_estimate" integer NULL,
+ "lane_6_cluster_estimate" integer NULL,
+ "lane_7_cluster_estimate" integer NULL,
+ "lane_8_cluster_estimate" integer NULL,
+ "cluster_mac_id" varchar(50) NOT NULL,
+ "seq_mac_id" varchar(50) NOT NULL,
+ "notes" text NOT NULL
+);''')
+ c.execute('''insert into experiments_flowcell
+ (id, flowcell_id, run_date, advanced_run, paired_end, read_length,
+ lane_1_library_id, lane_2_library_id, lane_3_library_id,
+ lane_4_library_id, lane_5_library_id, lane_6_library_id,
+ lane_7_library_id, lane_8_library_id, lane_1_pm,
+ lane_2_pM, lane_3_pM, lane_4_pM, lane_5_pM, lane_6_pM,
+ lane_7_pM, lane_8_pM, lane_1_cluster_estimate,
+ lane_2_cluster_estimate, lane_3_cluster_estimate,
+ lane_4_cluster_estimate, lane_5_cluster_estimate,
+ lane_6_cluster_estimate, lane_7_cluster_estimate,
+ lane_8_cluster_estimate, cluster_mac_id, seq_mac_id,
+ notes)
+ select
+ id, flowcell_id, run_date, advanced_run, paired_end, read_length,
+ lane_1_library_id, lane_2_library_id, lane_3_library_id,
+ lane_4_library_id, lane_5_library_id, lane_6_library_id,
+ lane_7_library_id, lane_8_library_id, lane_1_pm,
+ lane_2_pM, lane_3_pM, lane_4_pM, lane_5_pM, lane_6_pM,
+ lane_7_pM, lane_8_pM, lane_1_cluster_estimate,
+ lane_2_cluster_estimate, lane_3_cluster_estimate,
+ lane_4_cluster_estimate, lane_5_cluster_estimate,
+ lane_6_cluster_estimate, lane_7_cluster_estimate,
+ lane_8_cluster_estimate, "", "",
+ notes from fctracker_flowcell;''')
+ c.execute('''drop table fctracker_flowcell;''')
+
+ # create samples.cellline
+ c.execute('''CREATE TABLE "samples_cellline" (
+ "id" integer NOT NULL PRIMARY KEY,
+ "cellline_name" varchar(100) NOT NULL UNIQUE,
+ "nickname" varchar(20) NULL,
+ "notes" text NOT NULL);''')
+ c.execute('''insert into samples_cellline (cellline_name,notes) values("Unknown","Unknown");''')
+
+ # Create samples.condition
+ c.execute('''CREATE TABLE "samples_condition" (
+ "id" integer NOT NULL PRIMARY KEY,
+ "condition_name" varchar(2000) NOT NULL UNIQUE,
+ "nickname" varchar(20) NULL,
+ "notes" text NOT NULL);''')
+ c.execute('''insert into samples_condition (condition_name,notes) values("Unknown","Unknown");''')
+
+ # create samples.library
+ c.execute('''CREATE TABLE "samples_library" (
+ "id" integer NOT NULL PRIMARY KEY,
+ "library_id" varchar(30) NOT NULL,
+ "library_name" varchar(100) NOT NULL UNIQUE,
+ "library_species_id" integer NOT NULL REFERENCES "samples_species" ("id"),
+ "cell_line_id" integer NOT NULL REFERENCES "samples_cellline" ("id"),
+ "condition_id" integer NOT NULL REFERENCES "samples_condition" ("id"),
+ "antibody_id" integer NULL REFERENCES "samples_antibody" ("id"),
+ "replicate" smallint unsigned NOT NULL,
+ "experiment_type" varchar(50) NOT NULL,
+ "creation_date" date NULL,
+ "made_for" varchar(50) NOT NULL,
+ "made_by" varchar(50) NOT NULL,
+ "stopping_point" varchar(25) NOT NULL,
+ "amplified_from_sample_id" integer NULL,
+ "undiluted_concentration" decimal NULL,
+ "successful_pM" decimal NULL,
+ "ten_nM_dilution" bool NOT NULL,
+ "avg_lib_size" integer NULL,
+ "notes" text NOT NULL);''')
+ c.execute('''INSERT INTO samples_library
+ (id,library_id,library_name,library_species_id, experiment_type,
+ cell_line_id,condition_id,replicate,made_by,creation_date,
+ made_for,stopping_point,amplified_from_sample_id,
+ undiluted_concentration,ten_nM_dilution,successful_pM,
+ avg_lib_size,notes)
+select library_id,library_id,library_name,library_species_id,"unknown",
+ 1, 1, 1, made_by,creation_date,
+ made_for,stopping_point,amplified_from_sample_id,
+ undiluted_concentration,ten_nM_dilution,successful_pM,
+ 0,notes from fctracker_library;''');
+ c.execute('''update samples_library set experiment_type="RNA-seq" where library_id in (select library_id from fctracker_library where RNASeq = 1);''')
+ #c.execute('''drop table fctracker_library;''')
+ # add many to many tables
+ c.execute('''CREATE TABLE "samples_library_affiliations" (
+ "id" integer NOT NULL PRIMARY KEY,
+ "library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "affiliation_id" integer NOT NULL REFERENCES "samples_affiliation" ("id"),
+ UNIQUE ("library_id", "affiliation_id"));''')
+
+ c.execute('''CREATE TABLE "samples_library_tags" (
+ "id" integer NOT NULL PRIMARY KEY,
+ "library_id" integer NOT NULL REFERENCES "samples_library" ("id"),
+ "tag_id" integer NOT NULL REFERENCES "samples_tag" ("id"),
+ UNIQUE ("library_id", "tag_id"));''')
+
+
+
+ #
+ c.execute('''CREATE TABLE "samples_species" (
+ "id" integer NOT NULL PRIMARY KEY,
+ "scientific_name" varchar(256) NOT NULL,
+ "common_name" varchar(256) NOT NULL);''')
+ c.execute('''insert into samples_species
+ (id, scientific_name, common_name)
+ select
+ id, scientific_name, common_name
+ from fctracker_species;''')
+ c.execute('''drop table fctracker_species''')
+ conn.commit()
+
+if __name__ == "__main__":
+ main(sys.argv[1:])