GNU Scientific Library (gsl)
Used for descrptiv statistics: mean/variance/etc
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+chr17_random 28908 1
+chr17_random 28147 0
+chr17_random 28684 0
+chr17_random 48554 1
+chr17_random 141189 0
+chr17_random 183657 0
+chr17_random 193044 0
+chr17_random 200726 0
+chr17_random 342078 0
+chr17_random 346855 0
+chr17_random 354408 0
+chr17_random 373536 0
+chr17_random 375655 0
+chr17_random 581194 0
+chrUn_random 4452 1
+chrUn_random 21008 1
+chrUn_random 38715 1
+chrUn_random 127960 1
+chrUn_random 280356 1
+chrUn_random 528969 1
+chrUn_random 551814 1
+chrUn_random 551804 0
+chrUn_random 552839 0
+chrUn_random 552990 0
+chrUn_random 553286 0
+chrUn_random 553318 0
+chrUn_random 588617 1
+chrUn_random 554255 0
+chrUn_random 554532 0
+chrUn_random 555721 0
+chrUn_random 556017 0
+chrUn_random 556049 0
+chrUn_random 556255 0
+chrUn_random 588638 0
+chrUn_random 609525 1
+chrUn_random 609763 1
+chrUn_random 610174 1
+chrUn_random 610943 1
+chrUn_random 611252 1
+chrUn_random 616600 1
+chrUn_random 616889 1
+chrUn_random 617250 1
+chrUn_random 617640 0
+chrUn_random 618018 1
+chrUn_random 618327 1
+chrUn_random 618310 0
+chrUn_random 677715 1
+chrUn_random 740293 1
+chrUn_random 748660 0
+chrUn_random 748969 0
+chrUn_random 749696 0
+chrUn_random 750107 0
+chrUn_random 751530 0
+chrUn_random 752095 0
+chrUn_random 752685 0
+chrUn_random 752980 0
+chrUn_random 754430 1
+chrUn_random 754413 0
+chrUn_random 754722 0
+chrUn_random 882572 1
+chrUn_random 1102368 0
+chrUn_random 1102368 0
+chrUn_random 1383600 0
+chrUn_random 1585650 0
+chrUn_random 1691372 1
+chrUn_random 1737772 0
+chrUn_random 1957721 1
+chrUn_random 1957721 1
+chrUn_random 2216875 1
+chrUn_random 2334215 0
+chrUn_random 2553639 0
+chrUn_random 2560072 0
+chrUn_random 2572768 0
+chrUn_random 2678403 1
+chrUn_random 2735832 0
+chrUn_random 2735832 0
+chrUn_random 2891056 1
+chrUn_random 3058602 0
+chrUn_random 3837247 1
+chrUn_random 4677187 1
+chrUn_random 4834599 0
--- /dev/null
+#!/usr/bin/perl -w
+
+use strict;
+use warnings;
+
+my @libs = `ls -1d Flowcells/**/*.align*.txt`;
+
+my %libraries;
+
+for my $filename (@libs) {
+ chomp $filename;
+
+ my $base_file = `basename $filename`;
+ chomp $base_file;
+ $base_file =~ /^(\d+)_(.+?)_s(\d+)_.+?([Ss][Ll]\d+)\.align_.*/;
+ my($date,$flowcell,$lanes,$lib) = ($1,$2,$3,$4);
+
+ open(COUNT,$filename.".count");
+ my $count = <COUNT>; chomp $count;
+ if(!defined($count)) { print STDERR $filename,"\n"; }
+ close(COUNT);
+
+ if(!defined($lib)) {
+ print STDERR "MISSING LIB: ", $filename,"\n";
+ }
+
+ if(!exists($libraries{$lib})) { my @a; $libraries{$lib} = \@a; }
+ push @{$libraries{$lib}}, "$flowcell\t$lanes\t$filename\t$count\t$date";
+}
+
+ print "<?xml version=\"1.0\" ?>\n";
+ print "<Libraries>\n";
+
+ for my $lib (sort {$a =~ /[sS][lL](\d+)/; my $a1 = $1; $b =~ /[sS][lL](\d+)/; my $b1 = $1; $a1 <=> $b1} keys %libraries) {
+ print "<Library Name=\"$lib\">\n";
+ for my $data (@{$libraries{$lib}}) {
+ my($f,$l,$fn,$N,$D) = split(/\t/,$data);
+ print "<Track Flowcell=\"$f\" Lane=\"$l\" Filename=\"$fn\" Count=\"$N\" Date=\"$D\" />\n";
+ }
+ print "</Library>\n";
+ }
+
+ print "</Libraries>\n";
+
#
ROOT_DIR=/Users/ENCODE/svn_test/htsworkflow/htswanalysis
DATA_DIR=/Users/Data/
+HTML_DIR=/Library/WebServer/Documents/SequencingSummaries/
FILES=$(shell ls -1d $(DATA_DIR)/Flowcells/**/*.align*.txt)
QPCR_FILES=$(shell ls -1d $(DATA_DIR)/Flowcells/**/*.align*.txt | sed -e s/txt/txt.qPCR/)
$(ROOT_DIR)/bin/qPCR $(subst .txt.qPCR,.txt,$@) $(ROOT_DIR)/reference_data/GenericBackground $(ROOT_DIR)/reference_data/qPCR_Tests/ | sort -k 2 -g -r | perl -e> $@
%.txt.profile: %.txt
- $(ROOT_DIR)/profile_reads/profile_reads_against_features `echo $@ | sed -e s/\.profile//` $(ROOT_DIR)/profile_reads/`basename $@ | awk -F\. '{ print $$3 }'`_tx_start_sites > $@
+ $(ROOT_DIR)/profile_reads/profile_reads_against_features `echo $@ | sed -e s/\.profile//` $(ROOT_DIR)/reference_data/`basename $@ | awk -F\. '{ print $$3 }'`_tx_start_sites > $@
$(ROOT_DIR)/profile_reads/profile_to_svg.pm $@ > $@.svg
Libraries/%.txt: Libraries/.%.config | LibraryInfo.xml
#cat qPCR_summary.txt | ~/Summarize_qPCR.pm > qPCR_summary.html
LibraryInfo.xml: $(COUNT_FILES) $(CMPLX_FILES)
- $(ROOT_DIR)/CollectLibraries.pm > LibraryInfo.xml
- $(ROOT_DIR)/RecompileLibraries.pm LibraryInfo.xml
+ $(ROOT_DIR)/scripts/CollectLibraries.pm > LibraryInfo.xml
+ $(ROOT_DIR)/scripts/RecompileLibraries.pm LibraryInfo.xml
SequencingSummary.html: LibraryInfo.xml
- $(ROOT_DIR)/SummarizeLibrary.pm LibraryInfo.xml > SequencingSummary.html
+ $(ROOT_DIR)/scripts/SummarizeLibrary.pm LibraryInfo.xml > SequencingSummary.html
#SequencingLaneSummary.html: LibraryInfo.xml
#~/SummarizeLanes.pm LibraryInfo.xml > SequencingLaneSummary.html
#Distribute: SequencingSummary.html qPCR_summary.html SequencingLaneSummary.html
Distribute: SequencingSummary.html
- cp $^ /Library/WebServer/Documents/SequencingSummaries/;
+ cp $^ $(HTML_DIR);
Libraries: $(LIBFILES)
--- /dev/null
+#!/usr/bin/perl -w
+
+use strict;
+use warnings;
+
+use XML::Simple;
+
+my $REPODIR = '/Users/Data/Libraries';
+
+my $xml_file = shift;
+my $xml = XMLin($xml_file, ForceArray => 1);
+
+my @new_libraries;
+my @updated_libraries;
+
+for my $lib (@{$xml->{Library}}) {
+ my @files;
+ my $libname = uc($lib->{Name});
+ for my $track (@{$lib->{Track}}) {
+ my($date,$fc,$lane,$desc);
+ push @files, $track->{Filename};
+ }
+ my @sorted_files = sort {$a cmp $b} @files;
+
+ my $config_filename = "$REPODIR/.$libname.config";
+ if(-e $config_filename) {
+ my $idx = 0;
+ open OLD_CONFIG, $config_filename;
+ my @old_config = <OLD_CONFIG>;
+ close(OLD_CONFIG);
+
+ if(scalar(@old_config) < scalar(@sorted_files)) {
+ push @updated_libraries, "$config_filename";
+ open CONFIG, ">$config_filename";
+ for(@sorted_files) { print CONFIG "$_\n"; }
+ close(CONFIG);
+ } elsif(scalar(@old_config) > scalar(@sorted_files)) {
+ push @updated_libraries, "$config_filename";
+ open CONFIG, ">$config_filename";
+ for(@sorted_files) { print CONFIG "$_\n"; }
+ close(CONFIG);
+ } else {
+ for(0..scalar(@old_config)-1) {
+ my $track_line = $old_config[$_]; chomp $track_line;
+ if($track_line ne $sorted_files[$_]) {
+ push @updated_libraries, "$config_filename";
+ open CONFIG, ">$config_filename";
+ for(@sorted_files) { print CONFIG "$_\n"; }
+ close(CONFIG);
+ last;
+ }
+ }
+ }
+ } else {
+ push @new_libraries, "$config_filename";
+ open CONFIG, ">$config_filename";
+ for(@sorted_files) { print CONFIG "$_\n"; }
+ close(CONFIG);
+ }
+}
+
+print "===== New Libraries =====\n";
+for(@new_libraries) { print $_,"\n"; }
+print "===== Updated Libraries =====\n";
+for(@updated_libraries) { print $_,"\n"; }
+
+ #my $filename = $xml->{Library}->[$i]->{Track}->[$t]->{Filename};
+ #$filename =~ /^(\d+)_(.+?)_s(\d+)_(.+?)_$lib.align/;
+ #($date,$fc,$lane,$desc) = ($1,$2,$3,$4);
+ #$num_lanes += length($lane);
+ #$num_reads += $xml->{Library}->[$i]->{Track}->[$t]->{Count};
+ #}
+#}
--- /dev/null
+#!/usr/bin/perl -w
+
+use strict;
+use warnings;
+
+use XML::Simple;
+
+my $filename = shift;
+my $standalone = shift;
+
+my %month;
+$month{0} = "Jan";
+$month{1} = "Jan";
+$month{2} = "Feb";
+$month{3} = "Mar";
+$month{4} = "Apr";
+$month{5} = "May";
+$month{6} = "Jun";
+$month{7} = "Jul";
+$month{8} = "Aug";
+$month{9} = "Sep";
+$month{10} = "Oct";
+$month{11} = "Nov";
+$month{12} = "Dec";
+
+if(defined($standalone)) { print "<HTML><HEAD><TITLE>Sequencing Summary</TITLE></HEAD><BODY>\n"; }
+
+my $total_reads = 0;
+my $summary = SummarizeLibrary($filename);
+
+printf ("<H1>Solexa Sequencing Summary</H1><H2>Over %0.2f Billion Bases (%0.2f Human Genomes) Served</H2>\n",25*$total_reads/1e9,25*$total_reads/3.3e9);
+
+print $summary;
+
+if(defined($standalone)) { print "</BODY></HTML>\n"; }
+
+sub SummarizeLibrary {
+ my $xml_file = shift;
+ my $xml = XMLin($xml_file, ForceArray => 1);
+
+ my $summary = "";
+
+ $summary .= "<TABLE BORDER=1><TR><TD><EM>Library</EM></TD><TD><EM>Name</EM></TD><TD><EM>Num Lanes</EM></TD><TD><EM>Num Reads</EM></TD></TR>\n";
+
+ for my $x (0..scalar(@{$xml->{Library}})-1) {
+ my $i = scalar(@{$xml->{Library}})-1-$x;
+ my $lib = $xml->{Library}->[$i]->{Name};
+ my $N = scalar(@{$xml->{Library}->[$i]->{Track}});
+ my($date,$fc,$lane,$desc);
+ my $num_reads = 0;
+ my $num_lanes = 0;
+
+ my($start_date,$end_date);
+ for my $t (0..$N-1) {
+ my $filename = `basename $xml->{Library}->[$i]->{Track}->[$t]->{Filename}`;
+ $filename =~ /^(\d+)_(.+?)_s(\d+)_(.+?)_$lib.align/;
+ ($date,$fc,$lane,$desc) = ($1,$2,$3,$4);
+ $num_lanes += length($lane);
+ $num_reads += $xml->{Library}->[$i]->{Track}->[$t]->{Count};
+ if(!defined($start_date)) {
+ $start_date = $date;
+ $end_date = $date;
+ }
+
+ if($date < $start_date) { $start_date = $date; }
+ if($date > $end_date) { $end_date = $date; }
+ }
+
+ my $bgcolor;
+ if($num_reads < 3000000) { $bgcolor = "FF3300"; }
+ elsif($num_reads < 5000000) { $bgcolor = "FFCC33"; }
+ elsif($num_reads < 10000000) { $bgcolor = "00CCFF"; }
+ else { $bgcolor = "66FF66"; }
+
+ $total_reads += $num_reads;
+
+ $start_date =~ /(\d\d)(\d\d)(\d\d)/;
+ my $start_year = 2000+$1; my $start_mo = $month{int($2)}; my $start_day = $3;
+
+ $end_date =~ /(\d\d)(\d\d)(\d\d)/;
+ my $end_year = 2000+$1; my $end_mo = $month{int($2)}; my $end_day = $3;
+
+ if($start_day == 0) { $start_day = 1; } if($end_day == 0) { $end_day = 1; }
+ $summary .= sprintf("<TR BGCOLOR=#%s><TD>%s</TD><TD>%s</TD><TD>%d</TD><TD>%0.2fM</TD><TD>%s %d, %d</TD><TD>%s %d, %d</TR>\n",$bgcolor,$lib,$desc,$num_lanes,$num_reads/1000000.0,$start_mo,$start_day,$start_year,$end_mo,$end_day,$end_year);
+ }
+
+ $summary .= "</TABLE>\n";
+
+ return $summary;
+}
CPPFLAGS=-g -Wall -O3 -I/opt/local/include
LDFLAGS=-lgsl -lgslcblas -lm -L/opt/local/lib
-TARGETS=complexity_count qPCR
+TARGETS=complexity_count qPCR profile_reads_against_features
all: $(TARGETS)
--- /dev/null
+#include <iostream>
+#include <fstream>
+#include <vector>
+#include <queue>
+#include <math.h>
+#include <string>
+
+#define WINDOW 25
+
+using namespace std;
+
+vector<int> profile(2000,0);
+
+void int_to_chromstr(int chrom, char* chr);
+int chromstr_to_int(string chrom);
+
+void split (const string& text, const string& separators, vector<string>& words);
+
+class Hit {
+ public:
+ int chr;
+ unsigned int pos;
+ bool strand;
+
+ Hit(int chr, unsigned int pos, bool strand) {
+ this->chr = chr; this->pos = pos; this->strand = strand;
+ }
+};
+
+typedef vector<Hit> Hits;
+
+bool compare_hits(const Hit &a, const Hit &b)
+{
+ if(a.chr == b.chr) {
+ return a.pos < b.pos;
+ } else {
+ return a.chr < b.chr;
+ }
+}
+
+int main(int argc, char** argv) {
+ if(argc < 2) { cerr << "Usage: " << argv[0] << " [aligned reads filename] [file with list of feature starts]\n"; exit(0); }
+ char filename[128]; strcpy(filename,argv[1]);
+ char feature_filename[128]; strcpy(feature_filename,argv[2]);
+ Hits data;
+
+ Hits features;
+
+ string delim("\t");
+ string location_delim(":");
+
+ ifstream feat(feature_filename);
+ size_t N = 0;
+ while(feat.peek() != EOF) {
+ char line[1024];
+ feat.getline(line,1024,'\n');
+ N++;
+ string line_str(line);
+ vector<string> fields;
+ split(line_str, delim, fields);
+ if(fields.size() != 3) { cerr << "Error: wrong number of fields in feature list (line " << N << " has " << fields.size() << " fields)\n"; }
+
+ int chr = chromstr_to_int(fields[0]);
+ if(chr == -1) { continue; }
+ int pos = atoi(fields[1].c_str());
+ bool strand = (bool)(atoi(fields[2].c_str()));
+
+ Hit feat(chr,pos,strand);
+ features.push_back(feat);
+ }
+
+ cerr << "Found " << features.size() << " features\n";
+
+ //sort the features so we can run through it once
+ std::sort(features.begin(),features.end(),compare_hits);
+
+ delim = " \n";
+ location_delim = ":";
+ char strand_str[2]; strand_str[1] = '\0';
+ ifstream seqs(filename);
+ while(seqs.peek() != EOF) {
+ char line[2048];
+ seqs.getline(line,2048,'\n');
+ string line_str(line);
+ vector<string> fields;
+ split(line_str, delim, fields);
+ if(fields.size() == 3) { continue; }
+
+ vector<string> location; split(fields[3], location_delim, location);
+ int chr = chromstr_to_int(location[0]);
+ if(chr == -1) { continue; }
+ int pos = atoi(location[1].c_str());
+ bool strand = ((fields[4].c_str())[0] == 'F')?0:1;
+
+ Hit hit(chr,pos,strand);
+ data.push_back(hit);
+ }
+ seqs.close();
+
+ cerr << "Found " << data.size() << " reads\n";
+
+ //sort the data so we can run through it once
+ std::sort(data.begin(),data.end(),compare_hits);
+
+ int chrom = -1;
+ unsigned int feat_idx = 0;
+ int num_hits = 0;
+ for(Hits::iterator i = data.begin(); i != data.end(); ++i) {
+ num_hits++;
+ if(chrom == -1 || i->chr != chrom) {
+ chrom = i->chr;
+ feat_idx = 0;
+ while(feat_idx < features.size() && features[feat_idx].chr != chrom) { feat_idx++; }
+ if(feat_idx == features.size()) { break; }
+ cerr << chrom << " feat_idx: " << feat_idx << endl;
+ }
+
+ int dist_to_feature = i->pos - features[feat_idx].pos;
+ //if we have passed the last feature, fast forward to the next
+ while( feat_idx < features.size() && ((features[feat_idx].strand == 0 && dist_to_feature > 1000) || (features[feat_idx].strand == 1 && dist_to_feature > 1000))) {
+ if(features[feat_idx].chr != i->chr ) { goto end_loop; }
+ feat_idx++;
+ dist_to_feature = i->pos - features[feat_idx].pos;
+ }
+
+ if(features[feat_idx].strand == 1) { dist_to_feature *= -1; }
+
+ if(dist_to_feature > -1000 && dist_to_feature < 1000) {
+ profile[dist_to_feature + 1000]++;
+ }
+ end_loop:
+ ;
+ }
+
+ for(unsigned int i = 0; i < profile.size(); i++) {
+ cout << (int)i - (int)1000 << "\t" << (double)profile[i] / (double)data.size() << endl;;
+ }
+}
+
+int chromstr_to_int(string chromstr) {
+ const char* chrom = chromstr.c_str();
+ if(chrom[0] != 'c' || chrom[1] != 'h' || chrom[2] != 'r') { return -1; }
+ if(chrom[3] == 'X') { return 23; } else
+ if(chrom[3] == 'Y') { return 24; } else
+ if(chrom[3] == 'M') { return 25; } else
+ if(chrom[3] >= '0' && chrom[3] <= '9') { return atoi(chrom+3); } else
+ { return -1; }
+}
+
+void int_to_chromstr(int chrom, char* chr) {
+ strcpy(chr,"chr\0\0\0");
+ switch(chrom) {
+ case 23: chr[3]='X'; break;
+ case 24: chr[3]='Y'; break;
+ case 25: chr[3]='M'; break;
+ default: chr[3]=(char)(48+(int)(chrom/10));
+ chr[4]=(char)(48+(int)(chrom - 10*(int)(chrom/10)));
+ if(chr[3]=='0') { chr[3]=chr[4]; chr[4]='\0'; }
+ }
+}
+
+
+void split (const string& text, const string& separators, vector<string>& words) {
+
+ size_t n = text.length ();
+ size_t start = text.find_first_not_of (separators);
+
+ while (start < n) {
+ size_t stop = text.find_first_of (separators, start);
+ if (stop > n) stop = n;
+ words.push_back (text.substr (start, stop-start));
+ start = text.find_first_not_of (separators, stop+1);
+ }
+}