// ---------- mussa_class.cc -----------
// ----------------------------------------
+#include <boost/filesystem/path.hpp>
#include <boost/filesystem/operations.hpp>
#include <boost/filesystem/fstream.hpp>
namespace fs = boost::filesystem;
#include <sstream>
#include "mussa_exceptions.hpp"
-#include "alg/mussa.hpp"
#include "alg/flp.hpp"
+#include "alg/mussa.hpp"
+#include "alg/motif_parser.hpp"
using namespace std;
: color_mapper(new AnnotationColors)
{
clear();
- connect(&the_paths, SIGNAL(progress(const std::string&, int, int)),
- this, SIGNAL(progress(const std::string&, int, int)));
+ connect(&the_paths, SIGNAL(progress(const QString&, int, int)),
+ this, SIGNAL(progress(const QString&, int, int)));
}
Mussa::Mussa(const Mussa& m)
thres_append(m.thres_append),
motif_sequences(m.motif_sequences),
color_mapper(m.color_mapper),
+ analysis_path(m.analysis_path),
dirty(m.dirty)
{
- connect(&the_paths, SIGNAL(progress(const std::string&, int, int)),
- this, SIGNAL(progress(const std::string&, int, int)));
+ connect(&the_paths, SIGNAL(progress(const QString&, int, int)),
+ this, SIGNAL(progress(const QString&, int, int)));
+}
+
+MussaRef Mussa::init()
+{
+ boost::shared_ptr<Mussa> m(new Mussa());
+ return m;
+}
+
+boost::filesystem::path Mussa::get_analysis_path() const
+{
+ return analysis_path;
+}
+
+void Mussa::set_analysis_path(boost::filesystem::path pathname)
+{
+ analysis_path = pathname;
}
// set all parameters to null state
thres_append = false;
motif_sequences.clear();
if(color_mapper) color_mapper->clear();
+ the_seqs.clear();
the_paths.clear();
- dirty = false;
+ analysis_path = fs::path();
+ set_dirty(false);
+}
+
+void Mussa::set_dirty(bool new_state)
+{
+ if (dirty != new_state) {
+ dirty = new_state;
+ emit isModified(dirty);
+ }
}
+bool Mussa::is_dirty() const
+{
+ return dirty;
+}
+
+bool Mussa::empty() const
+{
+ return the_seqs.empty();
+}
+
+
// these 5 simple methods manually set the parameters for doing an analysis
// used so that the gui can take input from user and setup the analysis
// note - still need a set_append(bool, bool) method...
Mussa::set_name(string a_name)
{
analysis_name = a_name;
- dirty = true;
+ set_dirty(true);
}
-string Mussa::get_name()
+string Mussa::get_name() const
{
return analysis_name;
}
+string Mussa::get_title() const
+{
+ fs::path analysis_path = get_analysis_path();
+ if (not analysis_path.empty()) {
+ return analysis_path.native_file_string();
+ } else if (get_name().size() > 0) {
+ return get_name();
+ } else {
+ return std::string("Unnamed");
+ }
+}
+
int
Mussa::size() const
{
Mussa::set_window(int a_window)
{
window = a_window;
- dirty = true;
+ set_dirty(true);
}
int Mussa::get_window() const
Mussa::set_threshold(int a_threshold)
{
threshold = a_threshold;
- dirty = true;
+ set_dirty(true);
if (a_threshold > soft_thres) {
soft_thres = a_threshold;
}
Mussa::set_analysis_mode(enum analysis_modes new_ana_mode)
{
ana_mode = new_ana_mode;
- dirty = true;
+ set_dirty(true);
}
enum Mussa::analysis_modes Mussa::get_analysis_mode() const
{
boost::shared_ptr<Sequence> seq_copy(new Sequence(a_seq));
the_seqs.push_back(seq_copy);
- dirty = true;
+ set_dirty(true);
}
void Mussa::append_sequence(boost::shared_ptr<Sequence> a_seq)
{
the_seqs.push_back(a_seq);
- dirty = true;
+ set_dirty(true);
}
-const vector<boost::shared_ptr<Sequence> >&
+const vector<SequenceRef>&
Mussa::sequences() const
{
return the_seqs;
aseq->set_species(*name);
}
the_seqs.push_back(aseq);
- dirty = true;
+ set_dirty(true);
}
void
// << " threshold = " << threshold << endl;
}
// no file was loaded, signal error
- dirty = true;
+ set_dirty(true);
}
throw mussa_analysis_error("you need to have at least 2 sequences to "
"do an analysis.");
}
- //cout << "nway ana: seq_num = " << the_seqs.size() << endl;
-
+
seqcomp();
the_paths.setup(window, threshold);
nway();
- // FIXME: once we implement a save feature we should remove this
- if (not analysis_name.empty()) {
- save();
- }
}
void
void
Mussa::save(fs::path save_path)
{
- fs::path flp_filepath;
fs::fstream save_file;
ostringstream append_info;
int dir_create_status;
if (save_path.empty()) {
- if (not analysis_name.empty()) {
+ if (not analysis_path.empty()) {
+ save_path = analysis_path;
+ } else if (not analysis_name.empty()) {
std::string save_name = analysis_name;
// gotta do bit with adding win & thres if to be appended
if (win_append) {
if (not fs::exists(save_path)) {
fs::create_directory(save_path);
}
+
+ std::string basename = save_path.leaf();
+ fs::path museq(basename + ".museq", fs::native);
+
// save sequence and annots to a special mussa file
- save_file.open(save_path / (save_path.leaf()+".museq"), ios::out);
+ save_file.open(save_path / museq, ios::out);
save_file << "<Mussa_Sequence>" << endl;
for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++)
save_file << "</Mussa_Sequence>" << endl;
save_file.close();
+ // if we have any motifs, save them.
+ if (motif_sequences.size()) {
+ fs::path mtl(basename + ".mtl", fs::native);
+ save_motifs(save_path / mtl);
+ }
+
// save nway paths to its mussa save file
- the_paths.save(save_path / (save_path.leaf()+ ".muway"));
+ fs::path muway(basename + ".muway", fs::native);
+ the_paths.save(save_path / muway);
for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++) {
for(vector<Sequence>::size_type i2 = i+1; i2 < the_seqs.size(); i2++)
{
append_info.str("");
append_info << "_sp_" << i << "v" << i2;
- all_comps[i][i2].save(save_path/(save_path.leaf()+append_info.str()+".flp"));
+ fs::path flp(basename+append_info.str()+".flp", fs::native);
+ all_comps[i][i2].save(save_path / flp);
}
}
- dirty = false;
+
+ set_dirty(false);
+ analysis_path = save_path;
}
void
vector<FLPs> empty_FLP_vector;
FLPs dummy_comp;
- //clog << "ana_file name " << ana_file.string() << endl;
+ analysis_path = ana_file;
analysis_name = ana_path.leaf();
- //clog << " ana_name " << analysis_name << endl;
- file_path_base = ana_path.branch_path() / analysis_name;
- a_file_path = file_path_base / (analysis_name + ".muway");
- //clog << " loading museq: " << a_file_path.string() << endl;
+ fs::path muway(analysis_name+".muway", fs::native);
+ a_file_path = analysis_path / muway;
the_paths.load(a_file_path);
// perhaps this could be more elegent, but at least this'll let
// us know what our threshold and window sizes were when we load a muway
int seq_num = the_paths.sequence_count();
- a_file_path = file_path_base / (analysis_name + ".museq");
+ fs::path museq(analysis_name + ".museq", fs::native);
+ a_file_path = analysis_path / museq;
// this is a bit of a hack due to C++ not acting like it should with files
for (i = 1; i <= seq_num; i++)
{
boost::shared_ptr<Sequence> tmp_seq(new Sequence);
- //clog << "mussa_class: loading museq frag... " << a_file_path.string() << endl;
tmp_seq->load_museq(a_file_path, i);
the_seqs.push_back(tmp_seq);
}
+ fs::path mtl(analysis_name + ".mtl", fs::native);
+ fs::path motif_file = analysis_path / mtl;
+ if (fs::exists(motif_file)) {
+ load_motifs(motif_file);
+ }
empty_FLP_vector.clear();
for(i = 0; i < seq_num; i++)
{
append_info.str("");
append_info << analysis_name << "_sp_" << i << "v" << i2 << ".flp";
//clog << append_info.str() << endl;
- a_file_path = file_path_base / append_info.str();
- //clog << "path " << a_file_path.string() << endl;
+ fs::path flp(append_info.str(), fs::native);
+ a_file_path = analysis_path / flp;
all_comps[i][i2].load(a_file_path);
- //clog << "real size = " << all_comps[i][i2].size() << endl;
}
}
}
-
void
Mussa::save_old()
{
{
motif_sequences.insert(motif);
color_mapper->appendInstanceColor("motif", motif.get_sequence(), color);
+ set_dirty(true);
}
void Mussa::set_motifs(const vector<Sequence>& motifs,
update_sequences_motifs();
}
-// I mostly split the ifstream out so I can use a stringstream to test it.
+void Mussa::load_motifs(fs::path filename)
+{
+ fs::ifstream f;
+ f.open(filename, ifstream::in);
+ load_motifs(f);
+}
+
void Mussa::load_motifs(std::istream &in)
{
- string seq;
- float red;
- float green;
- float blue;
-
- while(in.good())
- {
- in >> seq >> red >> green >> blue;
- // if we couldn't read this line 'cause we're like at the end of the file
- // try to exit the loop
- if (!in.good())
- break;
- try {
- seq = Sequence::motif_normalize(seq);
- } catch(motif_normalize_error e) {
- clog << "unable to parse " << seq << " skipping" << endl;
- clog << e.what() << endl;
- continue;
- }
- if (red < 0.0 or red > 1.0) {
- clog << "invalid red value " << red << ". must be in range [0..1]"
- << endl;
- continue;
- }
- if (green < 0.0 or green > 1.0) {
- clog << "invalid green value " << green << ". must be in range [0..1]"
- << endl;
- continue;
- }
- if (blue < 0.0 or blue > 1.0) {
- clog << "invalid blue value " << blue << ". must be in range [0..1]"
- << endl;
- continue;
- }
- if (motif_sequences.find(seq) == motif_sequences.end()) {
- // sequence wasn't found
- motif_sequences.insert(seq);
- Color c(red, green, blue);
- color_mapper->appendInstanceColor("motif", seq, c);
- } else {
- clog << "sequence " << seq << " was already defined skipping"
- << endl;
- continue;
- }
+ std::string data;
+ const char *alphabet = Alphabet::dna_cstr;
+ motif_parser::ParsedMotifs parsed_motifs(motif_sequences, color_mapper);
+
+ // slurp our data into a string
+ std::streamsize bytes_read = 1;
+ while (in.good() and bytes_read) {
+ const std::streamsize bufsiz=512;
+ char buf[bufsiz];
+ bytes_read = in.readsome(buf, bufsiz);
+ data.append(buf, buf+bytes_read);
}
+ parsed_motifs.parse(data);
update_sequences_motifs();
}
-void Mussa::load_motifs(fs::path filename)
+void Mussa::save_motifs(fs::path filename)
{
- fs::ifstream f;
- f.open(filename, ifstream::in);
- load_motifs(f);
+ fs::ofstream out_stream;
+ out_stream.open(filename, ofstream::out);
+ save_motifs(out_stream);
+}
+
+void Mussa::save_motifs(std::ostream& out)
+{
+ for(motif_set::iterator motif_i = motif_sequences.begin();
+ motif_i != motif_sequences.end();
+ ++motif_i)
+ {
+ out << motif_i->get_sequence() << " ";
+ if (motif_i->get_name().size() > 0) {
+ out << "\"" << motif_i->get_name() << "\" ";
+ }
+ out << color_mapper->lookup("motif", motif_i->get_sequence());
+ out << std::endl;
+ }
}
void Mussa::update_sequences_motifs()
projects / mussa.git / blobdiff