// ---------- mussa_class.cc -----------
// ----------------------------------------
-#include <fstream>
+#include <boost/filesystem/path.hpp>
+#include <boost/filesystem/operations.hpp>
+#include <boost/filesystem/fstream.hpp>
+namespace fs = boost::filesystem;
+
#include <iostream>
#include <sstream>
#include "mussa_exceptions.hpp"
-#include "alg/mussa.hpp"
#include "alg/flp.hpp"
+#include "alg/mussa.hpp"
+#include "alg/motif_parser.hpp"
using namespace std;
+
Mussa::Mussa()
+ : color_mapper(new AnnotationColors)
{
clear();
+ connect(&the_paths, SIGNAL(progress(const QString&, int, int)),
+ this, SIGNAL(progress(const QString&, int, int)));
}
Mussa::Mussa(const Mussa& m)
win_append(m.win_append),
thres_append(m.thres_append),
motif_sequences(m.motif_sequences),
- color_mapper(m.color_mapper)
+ color_mapper(m.color_mapper),
+ analysis_path(m.analysis_path),
+ dirty(m.dirty)
{
+ connect(&the_paths, SIGNAL(progress(const QString&, int, int)),
+ this, SIGNAL(progress(const QString&, int, int)));
+}
+
+MussaRef Mussa::init()
+{
+ boost::shared_ptr<Mussa> m(new Mussa());
+ return m;
+}
+
+boost::filesystem::path Mussa::get_analysis_path() const
+{
+ return analysis_path;
+}
+
+void Mussa::set_analysis_path(boost::filesystem::path pathname)
+{
+ analysis_path = pathname;
}
// set all parameters to null state
win_append = false;
thres_append = false;
motif_sequences.clear();
- color_mapper.clear();
+ if(color_mapper) color_mapper->clear();
+ the_seqs.clear();
+ the_paths.clear();
+ analysis_path = fs::path();
+ set_dirty(false);
+}
+
+void Mussa::set_dirty(bool new_state)
+{
+ if (dirty != new_state) {
+ dirty = new_state;
+ emit isModified(dirty);
+ }
}
+bool Mussa::is_dirty() const
+{
+ return dirty;
+}
+
+bool Mussa::empty() const
+{
+ return the_seqs.empty();
+}
+
+
// these 5 simple methods manually set the parameters for doing an analysis
// used so that the gui can take input from user and setup the analysis
// note - still need a set_append(bool, bool) method...
Mussa::set_name(string a_name)
{
analysis_name = a_name;
+ set_dirty(true);
}
-string Mussa::get_name()
+string Mussa::get_name() const
{
return analysis_name;
}
+string Mussa::get_title() const
+{
+ fs::path analysis_path = get_analysis_path();
+ if (not analysis_path.empty()) {
+ return analysis_path.native_file_string();
+ } else if (get_name().size() > 0) {
+ return get_name();
+ } else {
+ return std::string("Unnamed");
+ }
+}
+
int
Mussa::size() const
{
Mussa::set_window(int a_window)
{
window = a_window;
+ set_dirty(true);
}
int Mussa::get_window() const
Mussa::set_threshold(int a_threshold)
{
threshold = a_threshold;
- //soft_thres = a_threshold;
+ set_dirty(true);
+ if (a_threshold > soft_thres) {
+ soft_thres = a_threshold;
+ }
}
int Mussa::get_threshold() const
}
void
-Mussa::set_soft_thres(int sft_thres)
+Mussa::set_soft_threshold(int new_threshold)
{
- soft_thres = sft_thres;
+ if (new_threshold < threshold) {
+ soft_thres = threshold;
+ } else if (new_threshold > window) {
+ soft_thres = window;
+ } else {
+ soft_thres = new_threshold;
+ }
}
-int Mussa::get_soft_thres() const
+int Mussa::get_soft_threshold() const
{
return soft_thres;
}
Mussa::set_analysis_mode(enum analysis_modes new_ana_mode)
{
ana_mode = new_ana_mode;
+ set_dirty(true);
}
enum Mussa::analysis_modes Mussa::get_analysis_mode() const
return the_paths;
}
-// takes a string and sets it as the next seq
-void
-Mussa::add_a_seq(string a_seq)
+//template <class IteratorT>
+//void Mussa::createLocalAlignment(IteratorT begin, IteratorT end)
+void Mussa::createLocalAlignment(std::list<ConservedPath>::iterator begin,
+ std::list<ConservedPath>::iterator end,
+ std::list<ConservedPath::path_type>& result,
+ std::list<std::vector<bool> >& reversed)
+{
+ const vector_sequence_type& raw_seq = the_seqs;
+ ConservedPath::path_type aligned_path;
+ size_t i2, i3;
+ int x_start, x_end;
+ int window_length, win_i;
+ int rc_1 = 0;
+ int rc_2 = 0;
+ vector<bool> rc_list;
+ bool full_match;
+ vector<bool> matched;
+ int align_counter;
+
+ align_counter = 0;
+ for(std::list<ConservedPath>::iterator pathz_i=begin; pathz_i != end; ++pathz_i)
+ {
+ ConservedPath& a_path = *pathz_i;
+ window_length = a_path.window_size;
+ // determine which parts of the path are RC relative to first species
+ rc_list = a_path.reverseComplimented();
+
+ // loop over each bp in the conserved region for all sequences
+ for(win_i = 0; win_i < window_length; win_i++)
+ {
+ aligned_path.clear();
+ // determine which exact base pairs match between the sequences
+ full_match = true;
+ for(i2 = 0; i2 < a_path.size()-1; i2++)
+ {
+ // assume not rc as most likely, adjust below
+ rc_1 = 0;
+ rc_2 = 0;
+ // no matter the case, any RC node needs adjustments
+ if (a_path[i2] < 0)
+ rc_1 = window_length-1;
+ if (a_path[i2+1] < 0)
+ rc_2 = window_length-1;
+
+ x_start = (abs(a_path[i2]-rc_1+win_i));
+ x_end = (abs(a_path[i2+1]-rc_2+win_i));
+
+ boost::shared_ptr<Sequence> cur(raw_seq[i2]) ;
+ boost::shared_ptr<Sequence> next(raw_seq[i2+1]);
+ // RC case handling
+ // ugh, and xor...only want rc coloring if just one of the nodes is rc
+ // if both nodes are rc, then they are 'normal' relative to each other
+ if((rc_list[i2] || rc_list[i2+1] )&&!(rc_list[i2] && rc_list[i2+1]))
+ { //the hideous rc matching logic - not complex, but annoying
+ if(!(( ((*cur)[x_start]=='A')&&((*next)[x_end]=='T')) ||
+ (((*cur)[x_start]=='T')&&((*next)[x_end]=='A')) ||
+ (((*cur)[x_start]=='G')&&((*next)[x_end]=='C')) ||
+ (((*cur)[x_start]=='C')&&((*next)[x_end]=='G'))) )
+ {
+ full_match = false;
+ } else {
+ aligned_path.push_back(x_start);
+ }
+ }
+ else
+ { // forward match
+ if (!( ((*cur)[x_start] == (*next)[x_end]) &&
+ ((*cur)[x_start] != 'N') && ((*next)[x_end] != 'N') ) ) {
+ full_match = false;
+ } else {
+ aligned_path.push_back(x_start);
+ }
+ }
+ }
+ // grab the last part of our path, assuming we matched
+ if (full_match)
+ aligned_path.push_back(x_end);
+
+ if (aligned_path.size() == a_path.size()) {
+ result.push_back(aligned_path);
+ reversed.push_back(rc_list);
+ }
+ }
+ align_counter++;
+ }
+}
+
+
+void Mussa::append_sequence(const Sequence& a_seq)
{
- Sequence aSeq;
+ boost::shared_ptr<Sequence> seq_copy(new Sequence(a_seq));
+ the_seqs.push_back(seq_copy);
+ set_dirty(true);
+}
- aSeq.set_seq(a_seq);
- the_seqs.push_back(aSeq);
+void Mussa::append_sequence(boost::shared_ptr<Sequence> a_seq)
+{
+ the_seqs.push_back(a_seq);
+ set_dirty(true);
}
-const vector<Sequence>&
+
+const vector<SequenceRef>&
Mussa::sequences() const
{
return the_seqs;
}
-void Mussa::load_sequence(string seq_file, string annot_file, int fasta_index,
- int sub_seq_start, int sub_seq_end)
+void Mussa::load_sequence(fs::path seq_file, fs::path annot_file,
+ int fasta_index, int sub_seq_start, int sub_seq_end,
+ std::string *name)
{
- Sequence aseq;
- aseq.load_fasta(seq_file, fasta_index, sub_seq_start, sub_seq_end);
- if (annot_file.size() > 0) {
- aseq.load_annot(annot_file, sub_seq_start, sub_seq_end);
+ boost::shared_ptr<Sequence> aseq(new Sequence);
+ aseq->load_fasta(seq_file, fasta_index, sub_seq_start, sub_seq_end);
+ if ( not annot_file.empty() ) {
+ aseq->load_annot(annot_file, sub_seq_start, sub_seq_end);
+ }
+ if (name != 0 and name->size() > 0 ) {
+ aseq->set_species(*name);
}
the_seqs.push_back(aseq);
+ set_dirty(true);
}
void
-Mussa::load_mupa_file(string para_file_path)
+Mussa::load_mupa_file(fs::path para_file_path)
{
- string file_path_base;
- ifstream para_file;
+ fs::ifstream para_file;
string file_data_line;
- string param, value, annot_file;
+ string param, value;
+ fs::path annot_file;
int split_index, fasta_index;
int sub_seq_start, sub_seq_end;
bool seq_params, did_seq;
// initialize values
clear();
- para_file.open(para_file_path.c_str(), ios::in);
-
// if file was opened, read the parameter values
- if (para_file)
+ if (not fs::exists(para_file_path))
{
- // need to find the path to the .mupa file
- parsing_path = true;
- dir_index = 0;
- while (parsing_path)
- {
- new_index = (para_file_path.substr(dir_index)).find("/");
- if (new_index != string::npos)
- dir_index += new_index + 1;
- else
- parsing_path = false;
- }
+ throw mussa_load_error("Config File: " + para_file_path.string() + " not found");
+ } else if (fs::is_directory(para_file_path)) {
+ throw mussa_load_error("Config File: " + para_file_path.string() + " is a directory.");
+ } else if (fs::is_empty(para_file_path)) {
+ throw mussa_load_error("Config File: " + para_file_path.string() + " is empty");
+ } else {
+ para_file.open(para_file_path, ios::in);
- file_path_base = para_file_path.substr(0,dir_index);
+ // what directory is the mupa file in?
+ fs::path file_path_base = para_file_path.branch_path();
// setup loop by getting file's first line
getline(para_file,file_data_line);
param = file_data_line.substr(0,split_index);
value = file_data_line.substr(split_index+1);
- while (!para_file.eof())
+ while (para_file)
{
did_seq = false;
if (param == "ANA_NAME")
threshold = atoi(value.c_str());
else if (param == "SEQUENCE")
{
- string seq_file = file_path_base + value;
+ fs::path seq_file = file_path_base / value;
//cout << "seq_file_name " << seq_files.back() << endl;
fasta_index = 1;
annot_file = "";
sub_seq_end = 0;
seq_params = true;
- while ((!para_file.eof()) && seq_params)
+ while (para_file && seq_params)
{
getline(para_file,file_data_line);
split_index = file_data_line.find(" ");
if (param == "FASTA_INDEX")
fasta_index = atoi(value.c_str());
else if (param == "ANNOTATION")
- annot_file = file_path_base + value;
+ annot_file = file_path_base / value;
else if (param == "SEQ_START")
sub_seq_start = atoi(value.c_str());
else if (param == "SEQ_END")
// << " threshold = " << threshold << endl;
}
// no file was loaded, signal error
- else
- {
- throw mussa_load_error("Config File: " + para_file_path + " not found");
- }
+ set_dirty(true);
}
void
-Mussa::analyze(int w, int t, enum Mussa::analysis_modes the_ana_mode, double new_ent_thres)
+Mussa::analyze()
{
- time_t t1, t2, begin, end;
- double seqloadtime, seqcomptime, nwaytime, savetime, totaltime;
-
- begin = time(NULL);
-
- ana_mode = the_ana_mode;
- ent_thres = new_ent_thres;
- if (w > 0)
- window = w;
- if (t > 0)
- {
- threshold = t;
- soft_thres = t;
- }
-
- t1 = time(NULL);
-
if (the_seqs.size() < 2) {
throw mussa_analysis_error("you need to have at least 2 sequences to "
"do an analysis.");
}
- //cout << "nway ana: seq_num = " << the_seqs.size() << endl;
-
- t2 = time(NULL);
- seqloadtime = difftime(t2, t1);
-
- t1 = time(NULL);
+
seqcomp();
- t2 = time(NULL);
- seqcomptime = difftime(t2, t1);
-
-
- t1 = time(NULL);
the_paths.setup(window, threshold);
nway();
- t2 = time(NULL);
- nwaytime = difftime(t2, t1);
-
- t1 = time(NULL);
- save();
- t2 = time(NULL);
- savetime = difftime(t2, t1);
-
- end = time(NULL);
- totaltime = difftime(end, begin);
-
-
- //cout << "seqload\tseqcomp\tnway\tsave\ttotal\n";
- //cout << seqloadtime << "\t";
- //cout << seqcomptime << "\t";
- //cout << nwaytime << "\t";
- //cout << savetime << "\t";
- //cout << totaltime << "\n";
}
void
for(vector<Sequence>::size_type i2 = 0; i2 < the_seqs.size(); i2++)
all_comps[i].push_back(dummy_comp);
}
- for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++)
- seq_lens.push_back(the_seqs[i].size());
+ for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++) {
+ seq_lens.push_back(the_seqs[i]->size());
+ }
+ int seqcomps_done = 0;
+ int seqcomps_todo = (the_seqs.size() * (the_seqs.size()-1)) / 2;
+ emit progress("seqcomp", seqcomps_done, seqcomps_todo);
for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++)
for(vector<Sequence>::size_type i2 = i+1; i2 < the_seqs.size(); i2++)
{
//cout << "seqcomping: " << i << " v. " << i2 << endl;
all_comps[i][i2].setup(window, threshold);
- all_comps[i][i2].seqcomp(the_seqs[i].get_seq(), the_seqs[i2].get_seq(), false);
- all_comps[i][i2].seqcomp(the_seqs[i].get_seq(),the_seqs[i2].rev_comp(),true);
+ all_comps[i][i2].seqcomp(*the_seqs[i], *the_seqs[i2], false);
+ all_comps[i][i2].seqcomp(*the_seqs[i], the_seqs[i2]->rev_comp(),true);
+ ++seqcomps_done;
+ emit progress("seqcomp", seqcomps_done, seqcomps_todo);
}
}
void
Mussa::nway()
{
- vector<string> some_Seqs;
- the_paths.set_soft_thres(soft_thres);
+ the_paths.set_soft_threshold(soft_thres);
if (ana_mode == TransitiveNway) {
the_paths.trans_path_search(all_comps);
}
else if (ana_mode == EntropyNway)
{
+ vector<Sequence> some_Seqs;
//unlike other methods, entropy needs to look at the sequence at this stage
some_Seqs.clear();
for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++)
{
- some_Seqs.push_back(the_seqs[i].get_seq());
+ some_Seqs.push_back(*the_seqs[i]);
}
the_paths.setup_ent(ent_thres, some_Seqs); // ent analysis extra setup
}
void
-Mussa::save()
+Mussa::save(fs::path save_path)
{
- string save_name, save_path, create_dir_cmd, flp_filepath;
- fstream save_file;
+ fs::fstream save_file;
ostringstream append_info;
int dir_create_status;
+ if (save_path.empty()) {
+ if (not analysis_path.empty()) {
+ save_path = analysis_path;
+ } else if (not analysis_name.empty()) {
+ std::string save_name = analysis_name;
+ // gotta do bit with adding win & thres if to be appended
+ if (win_append) {
+ append_info.str("");
+ append_info << "_w" << window;
+ save_name += append_info.str();
+ }
- // not sure why, but gotta close file each time since can't pass file streams
-
- save_name = analysis_name;
-
- // gotta do bit with adding win & thres if to be appended
- if (win_append)
- {
- append_info.str("");
- append_info << "_w" << window;
- save_name += append_info.str();
+ if (thres_append) {
+ append_info.str("");
+ append_info << "_t" << threshold;
+ save_name += append_info.str();
+ }
+ save_path = save_name;
+ } else {
+ throw mussa_save_error("Need filename or analysis name to save");
+ }
}
- if (thres_append)
- {
- append_info.str("");
- append_info << "_t" << threshold;
- save_name += append_info.str();
+ if (not fs::exists(save_path)) {
+ fs::create_directory(save_path);
}
-
-//#include <stdlib.h>
- // ******* use appropriate for os ------- 1 of 4
- // the additions for osX make it more sane where it saves the analysis
- // will come up with a cleaner sol'n later...
- create_dir_cmd = "mkdir " + save_name; //linux
- //create_dir_cmd = "mkdir " + file_path_base + save_name; //osX
-
- dir_create_status = system( (const char*) create_dir_cmd.c_str());
- //cout << "action: " << dir_create_status << endl;
-
+
+ std::string basename = save_path.leaf();
+ fs::path museq(basename + ".museq", fs::native);
+
// save sequence and annots to a special mussa file
-
- // ******** use appropriate for OS ---------- 2 of 4
- save_path = save_name + "/" + save_name + ".museq"; //linux
- //save_path = file_path_base + save_name + "/" + save_name + ".museq"; //osX
-
- save_file.open(save_path.c_str(), ios::out);
+ save_file.open(save_path / museq, ios::out);
save_file << "<Mussa_Sequence>" << endl;
- //save_file.close();
for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++)
{
- the_seqs[i].save(save_file);
+ the_seqs[i]->save(save_file);
}
- //save_file.open(save_path.c_str(), ios::app);
save_file << "</Mussa_Sequence>" << endl;
save_file.close();
- // save nway paths to its mussa save file
+ // if we have any motifs, save them.
+ if (motif_sequences.size()) {
+ fs::path mtl(basename + ".mtl", fs::native);
+ save_motifs(save_path / mtl);
+ }
- // ******** use appropriate for OS -------- 3 of 4
- save_path = save_name + "/" + save_name + ".muway"; //linux
- //save_path = file_path_base + save_name + "/" + save_name + ".muway"; //os X
- the_paths.save(save_path);
+ // save nway paths to its mussa save file
+ fs::path muway(basename + ".muway", fs::native);
+ the_paths.save(save_path / muway);
- for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++)
+ for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++) {
for(vector<Sequence>::size_type i2 = i+1; i2 < the_seqs.size(); i2++)
{
append_info.str("");
append_info << "_sp_" << i << "v" << i2;
- // ******** use appropriate for OS --------- 4 of 4
- //linux
- save_path = save_name + "/" + save_name + append_info.str() + ".flp";
- //osX
- //save_path = file_path_base + save_name + "/" + save_name + append_info.str() + ".flp";
- all_comps[i][i2].save(save_path);
+ fs::path flp(basename+append_info.str()+".flp", fs::native);
+ all_comps[i][i2].save(save_path / flp);
}
+ }
+
+ set_dirty(false);
+ analysis_path = save_path;
}
void
-Mussa::save_muway(string save_path)
+Mussa::save_muway(fs::path save_path)
{
the_paths.save(save_path);
}
void
-Mussa::load(string ana_file)
+Mussa::load(fs::path ana_file)
{
int i, i2;
- string::size_type start_index, end_index;
- string file_path_base, a_file_path, ana_path;
+ fs::path file_path_base;
+ fs::path a_file_path;
+ fs::path ana_path(ana_file);
bool parsing_path;
- Sequence tmp_seq;
string err_msg;
ostringstream append_info;
vector<FLPs> empty_FLP_vector;
FLPs dummy_comp;
- //cout << "ana_file name " << ana_file << endl;
- ana_path = ana_file;
- parsing_path = true;
- end_index = ana_path.size()-1;
- if (ana_path[end_index] == '/') {
- --end_index;
- }
- start_index = ana_path.rfind('/', end_index);
- if (start_index == string::npos) {
- // no / to be found
- start_index = 0;
- } else {
- // skip the / we found
- ++start_index;
- }
- analysis_name = ana_path.substr(start_index, end_index-start_index+1);
- //cout << " ana_name " << analysis_name << endl;
- file_path_base = ana_path.substr(0, start_index) + analysis_name
- + "/" + analysis_name;
- a_file_path = file_path_base + ".muway";
- //cout << " loading museq: " << a_file_path << endl;
+ analysis_path = ana_file;
+ analysis_name = ana_path.leaf();
+ fs::path muway(analysis_name+".muway", fs::native);
+ a_file_path = analysis_path / muway;
the_paths.load(a_file_path);
// perhaps this could be more elegent, but at least this'll let
// us know what our threshold and window sizes were when we load a muway
int seq_num = the_paths.sequence_count();
- a_file_path = file_path_base + ".museq";
+ fs::path museq(analysis_name + ".museq", fs::native);
+ a_file_path = analysis_path / museq;
// this is a bit of a hack due to C++ not acting like it should with files
for (i = 1; i <= seq_num; i++)
{
- tmp_seq.clear();
- //cout << "mussa_class: loading museq frag... " << a_file_path << endl;
- tmp_seq.load_museq(a_file_path, i);
+ boost::shared_ptr<Sequence> tmp_seq(new Sequence);
+ tmp_seq->load_museq(a_file_path, i);
the_seqs.push_back(tmp_seq);
}
+ fs::path mtl(analysis_name + ".mtl", fs::native);
+ fs::path motif_file = analysis_path / mtl;
+ if (fs::exists(motif_file)) {
+ load_motifs(motif_file);
+ }
empty_FLP_vector.clear();
for(i = 0; i < seq_num; i++)
{
for(i2 = i+1; i2 < seq_num; i2++)
{
append_info.str("");
- append_info << "_sp_" << i << "v" << i2;
- //cout << append_info.str() << endl;
- a_file_path = file_path_base + append_info.str() + ".flp";
+ append_info << analysis_name << "_sp_" << i << "v" << i2 << ".flp";
+ //clog << append_info.str() << endl;
+ fs::path flp(append_info.str(), fs::native);
+ a_file_path = analysis_path / flp;
all_comps[i][i2].load(a_file_path);
- //cout << "real size = " << all_comps[i][i2].size() << endl;
}
}
}
-
void
Mussa::save_old()
{
- fstream save_file;
+ fs::fstream save_file;
- save_file.open(analysis_name.c_str(), ios::out);
+ save_file.open(analysis_name, ios::out);
for(vector<Sequence>::size_type i = 0; i < the_seqs.size(); i++)
- save_file << the_seqs[i].get_seq() << endl;
+ save_file << *(the_seqs[i]) << endl;
save_file << window << endl;
save_file.close();
for(i = 0; i < seq_num; i++)
{
getline(save_file, file_data_line);
- a_seq.set_seq(file_data_line);
+ boost::shared_ptr<Sequence> a_seq(new Sequence(file_data_line));
the_seqs.push_back(a_seq);
}
//the_paths.save("tmp.save");
}
-void Mussa::add_motifs(const vector<string>& motifs,
+void Mussa::add_motif(const Sequence& motif, const Color& color)
+{
+ motif_sequences.insert(motif);
+ color_mapper->appendInstanceColor("motif", motif.get_sequence(), color);
+ set_dirty(true);
+}
+
+void Mussa::set_motifs(const vector<Sequence>& motifs,
const vector<Color>& colors)
{
if (motifs.size() != colors.size()) {
throw mussa_error("motif and color vectors must be the same size");
}
+ motif_sequences.clear();
for(size_t i = 0; i != motifs.size(); ++i)
{
- motif_sequences.insert(motifs[i]);
- color_mapper.appendInstanceColor("motif", motifs[i], colors[i]);
+ add_motif(motifs[i], colors[i]);
}
update_sequences_motifs();
}
-// I mostly split the ifstream out so I can use a stringstream to test it.
+void Mussa::load_motifs(fs::path filename)
+{
+ fs::ifstream f;
+ f.open(filename, ifstream::in);
+ load_motifs(f);
+}
+
void Mussa::load_motifs(std::istream &in)
{
- string seq;
- float red;
- float green;
- float blue;
-
- while(in.good())
- {
- in >> seq >> red >> green >> blue;
- // if we couldn't read this line 'cause we're like at the end of the file
- // try to exit the loop
- if (!in.good())
- break;
- try {
- seq = Sequence::motif_normalize(seq);
- } catch(motif_normalize_error e) {
- clog << "unable to parse " << seq << " skipping" << endl;
- clog << e.what() << endl;
- continue;
- }
- if (red < 0.0 or red > 1.0) {
- clog << "invalid red value " << red << ". must be in range [0..1]"
- << endl;
- continue;
- }
- if (green < 0.0 or green > 1.0) {
- clog << "invalid green value " << green << ". must be in range [0..1]"
- << endl;
- continue;
- }
- if (blue < 0.0 or blue > 1.0) {
- clog << "invalid blue value " << blue << ". must be in range [0..1]"
- << endl;
- continue;
- }
- if (motif_sequences.find(seq) == motif_sequences.end()) {
- // sequence wasn't found
- motif_sequences.insert(seq);
- Color c(red, green, blue);
- color_mapper.appendInstanceColor("motif", seq, c);
- } else {
- clog << "sequence " << seq << " was already defined skipping"
- << endl;
- continue;
- }
+ std::string data;
+ const char *alphabet = Alphabet::dna_cstr;
+ motif_parser::ParsedMotifs parsed_motifs(motif_sequences, color_mapper);
+
+ // slurp our data into a string
+ std::streamsize bytes_read = 1;
+ while (in.good() and bytes_read) {
+ const std::streamsize bufsiz=512;
+ char buf[bufsiz];
+ bytes_read = in.readsome(buf, bufsiz);
+ data.append(buf, buf+bytes_read);
}
+ parsed_motifs.parse(data);
update_sequences_motifs();
}
-void Mussa::load_motifs(string filename)
+void Mussa::save_motifs(fs::path filename)
{
- ifstream f;
- f.open(filename.c_str(), ifstream::in);
- load_motifs(f);
+ fs::ofstream out_stream;
+ out_stream.open(filename, ofstream::out);
+ save_motifs(out_stream);
+}
+
+void Mussa::save_motifs(std::ostream& out)
+{
+ for(motif_set::iterator motif_i = motif_sequences.begin();
+ motif_i != motif_sequences.end();
+ ++motif_i)
+ {
+ out << motif_i->get_sequence() << " ";
+ if (motif_i->get_name().size() > 0) {
+ out << "\"" << motif_i->get_name() << "\" ";
+ }
+ out << color_mapper->lookup("motif", motif_i->get_sequence());
+ out << std::endl;
+ }
}
void Mussa::update_sequences_motifs()
{
// once we've loaded all the motifs from the file,
// lets attach them to the sequences
- for(vector<Sequence>::iterator seq_i = the_seqs.begin();
+ for(vector<boost::shared_ptr<Sequence> >::iterator seq_i = the_seqs.begin();
seq_i != the_seqs.end();
++seq_i)
{
// clear out old motifs
- seq_i->clear_motifs();
+ (*seq_i)->clear_motifs();
// for all the motifs in our set, attach them to the current sequence
- for(set<string>::iterator motif_i = motif_sequences.begin();
+ for(set<Sequence>::iterator motif_i = motif_sequences.begin();
motif_i != motif_sequences.end();
++motif_i)
{
- seq_i->add_motif(*motif_i);
+ (*seq_i)->add_motif(*motif_i);
}
}
}
-const set<string>& Mussa::motifs() const
+const set<Sequence>& Mussa::motifs() const
{
return motif_sequences;
}
-AnnotationColors& Mussa::colorMapper()
+boost::shared_ptr<AnnotationColors> Mussa::colorMapper()
{
return color_mapper;
}