2 from django.db import models
3 from django.contrib.auth.models import User
4 from htsworkflow.frontend import settings
5 from htsworkflow.frontend.reports.libinfopar import *
7 # Create your models here.
9 class Antibody(models.Model):
10 antigene = models.CharField(max_length=500, db_index=True)
13 # alter table fctracker_antibody add column "nickname" varchar(20) NULL;
14 nickname = models.CharField(
19 verbose_name = 'Short Name'
21 catalog = models.CharField(max_length=50, unique=True, db_index=True)
22 antibodies = models.CharField(max_length=500, db_index=True)
23 source = models.CharField(max_length=500, blank=True, db_index=True)
24 biology = models.TextField(blank=True)
25 notes = models.TextField(blank=True)
26 def __unicode__(self):
27 return u'%s - %s (%s)' % (self.antigene, self.antibodies, self.catalog)
29 verbose_name_plural = "antibodies"
30 ordering = ["antigene"]
32 class Cellline(models.Model):
33 cellline_name = models.CharField(max_length=100, unique=True, db_index=True)
34 nickname = models.CharField(max_length=20,
38 verbose_name = 'Short Name')
39 notes = models.TextField(blank=True)
40 def __unicode__(self):
41 return unicode(self.cellline_name)
44 ordering = ["cellline_name"]
46 class Condition(models.Model):
47 condition_name = models.CharField(
48 max_length=2000, unique=True, db_index=True)
49 nickname = models.CharField(max_length=20,
53 verbose_name = 'Short Name')
54 notes = models.TextField(blank=True)
56 def __unicode__(self):
57 return unicode(self.condition_name)
60 ordering = ["condition_name"]
62 class ExperimentType(models.Model):
63 name = models.CharField(max_length=50, unique=True)
65 def __unicode__(self):
66 return unicode(self.name)
68 class Tag(models.Model):
69 tag_name = models.CharField(max_length=100, db_index=True,blank=False,null=False)
71 #('Antibody','Antibody'),
72 #('Cellline', 'Cellline'),
73 #('Condition', 'Condition'),
74 ('Library', 'Library'),
77 context = models.CharField(max_length=50,
78 choices=TAG_CONTEXT, default='Library')
80 def __unicode__(self):
81 return u'%s' % (self.tag_name)
84 ordering = ["context","tag_name"]
86 class Species(models.Model):
87 scientific_name = models.CharField(max_length=256,
91 common_name = models.CharField(max_length=256, blank=True)
92 #use_genome_build = models.CharField(max_length=100, blank=False, null=False)
94 def __unicode__(self):
95 return u'%s (%s)' % (self.scientific_name, self.common_name)
98 verbose_name_plural = "species"
99 ordering = ["scientific_name"]
101 class Affiliation(models.Model):
102 name = models.CharField(max_length=256, db_index=True, verbose_name='Group Name')
103 contact = models.CharField(max_length=256, null=True, blank=True,verbose_name='Contact Name')
104 email = models.EmailField(null=True,blank=True)
106 def __unicode__(self):
107 str = unicode(self.name)
108 if self.contact is not None and len(self.contact) > 0:
109 str += u' ('+self.contact+u')'
113 ordering = ["name","contact"]
114 unique_together = (("name", "contact"),)
116 class Library(models.Model):
117 id = models.AutoField(primary_key=True)
118 library_id = models.CharField(max_length=30, db_index=True)
119 library_name = models.CharField(max_length=100, unique=True)
120 library_species = models.ForeignKey(Species)
121 # new field 2008 Mar 5, alter table samples_library add column "hidden" NOT NULL default 0;
122 hidden = models.BooleanField()
123 cell_line = models.ForeignKey(Cellline)
124 condition = models.ForeignKey(Condition)
125 antibody = models.ForeignKey(Antibody,blank=True,null=True)
126 # New field Aug/25/08. SQL: alter table fctracker_library add column "lib_affiliation" varchar(256) NULL;
127 affiliations = models.ManyToManyField(Affiliation,related_name='library_affiliations',null=True)
128 # new field Nov/14/08
129 tags = models.ManyToManyField(Tag,related_name='library_tags',blank=True,null=True)
130 # New field Aug/19/08
131 # SQL to add column: alter table fctracker_library add column "replicate" smallint unsigned NULL;
132 REPLICATE_NUM = ((1,1),(2,2),(3,3),(4,4))
133 replicate = models.PositiveSmallIntegerField(choices=REPLICATE_NUM,default=1)
134 experiment_type = models.ForeignKey(ExperimentType)
135 creation_date = models.DateField(blank=True, null=True)
136 made_for = models.CharField(max_length=50, blank=True,
137 verbose_name='ChIP/DNA/RNA Made By')
138 made_by = models.CharField(max_length=50, blank=True, default="Lorian")
140 PROTOCOL_END_POINTS = (
142 ('Sample', 'Raw sample'),
143 ('Progress', 'In progress'),
144 ('1A', 'Ligation, then gel'),
145 ('PCR', 'Ligation, then PCR'),
146 ('1Ab', 'Ligation, PCR, then gel'),
147 ('1Aa', 'Ligation, gel, then PCR'),
148 ('2A', 'Ligation, PCR, gel, PCR'),
149 ('Done', 'Completed'),
151 stopping_point = models.CharField(max_length=25, choices=PROTOCOL_END_POINTS, default='Done')
152 amplified_from_sample = models.ForeignKey('self', blank=True, null=True)
154 undiluted_concentration = models.DecimalField("Concentration",
155 max_digits=5, decimal_places=2, default=0, blank=True, null=True,
156 help_text=u"Undiluted concentration (ng/\u00b5l)")
157 # note \u00b5 is the micro symbol in unicode
158 successful_pM = models.DecimalField(max_digits=9, decimal_places=1, blank=True, null=True)
159 ten_nM_dilution = models.BooleanField()
160 avg_lib_size = models.IntegerField(default=225, blank=True, null=True)
161 notes = models.TextField(blank=True)
163 def __unicode__(self):
164 return u'#%s: %s' % (self.library_id, self.library_name)
167 verbose_name_plural = "libraries"
168 ordering = ["-creation_date"] #["-library_id"]
170 def antibody_name(self):
171 str ='<a target=_self href="/admin/samples/antibody/'+self.antibody.id.__str__()+'/" title="'+self.antibody.__str__()+'">'+self.antibody.nickname+'</a>'
173 antibody_name.allow_tags = True
176 return self.library_species.common_name
178 def affiliation(self):
179 affs = self.affiliations.all().order_by('name')
183 ar.append(t.__unicode__())
184 return '%s' % (", ".join(ar))
187 affs = self.tags.all().order_by('tag_name')
190 ar.append(t.__unicode__())
191 return u'%s' % ( ", ".join(ar))
194 str ='<a target=_self href="/admin/experiments/datarun/?q='+self.library_id+'" title="Check All Data Runs for This Specific Library ..." ">Data Run</a>'
196 DataRun.allow_tags = True
198 def aligned_m_reads(self):
199 return getLibReads(self.library_id)
201 def aligned_reads(self):
202 res = getLibReads(self.library_id)
206 return u'<div style="border:solid red 2px">'+res[2]+'</div>'
208 rc = "%1.2f" % (res[1]/1000000.0)
209 # Color Scheme: green is more than 10M, blue is more than 5M, orange is more than 3M and red is less. For RNAseq, all those thresholds should be doubled
211 bgcolor = '#ff3300' # Red
212 rc_thr = [10000000,5000000,3000000]
213 if self.experiment_type == 'RNA-seq':
214 rc_thr = [20000000,10000000,6000000]
216 if res[1] > rc_thr[0]:
217 bgcolor = '#66ff66' # Green
219 if res[1] > rc_thr[1]:
220 bgcolor ='#00ccff' # Blue
222 if res[1] > rc_thr[2]:
223 bgcolor ='#ffcc33' # Orange
224 tstr = '<div style="background-color:'+bgcolor+';color:black">'
225 tstr += res[0].__unicode__()+' Lanes, '+rc+' M Reads'
227 else: tstr = 'not processed yet'
229 aligned_reads.allow_tags = True
233 summary_url = self.get_absolute_url()
234 return '<a href="%s">S</a>' % (summary_url,)
235 public.allow_tags = True
238 def get_absolute_url(self):
239 return ('htsworkflow.frontend.samples.views.library_to_flowcells', [str(self.id)])