1 # Lane 1 : Tile 1 : Raw : Read 1
2 4685939 bases of sequence found
5 1.20 percent error rate
6 0.18 percent blank rate
8 unique alignments : 126647 (total score 15145668)
11 Breakdown of errors by cycle
12 Cycle: Err pc: Blank pc:
51 Error rate relative to reference base (including blanks)
52 (Given a reference base, what is it sequenced as?)
55 N pc A pc C pc G pc T pc
56 A 0.18 97.92 1.25 0.39 0.26
57 C 0.18 0.23 98.95 0.23 0.41
58 G 0.18 0.29 0.27 98.80 0.47
59 T 0.19 0.17 0.51 0.23 98.89
61 Error rate relative to reference base (excluding blanks)
62 (Given a reference base, what is it sequenced as?)
66 A 98.09 1.25 0.39 0.26
67 C 0.23 99.13 0.23 0.41
68 G 0.30 0.27 98.97 0.47
69 T 0.17 0.51 0.23 99.08
71 Error rate relative to sequenced base (excluding ref blanks)
72 (Given a sequenced base, what was it really?)
76 N 26.16 24.03 23.42 26.39
77 A 99.34 0.22 0.28 0.17
78 C 1.35 97.86 0.26 0.52
79 G 0.42 0.23 99.12 0.24
80 T 0.28 0.40 0.46 98.87
82 Breakdown of errors by nucleotide
86 A 1212263 2648 3363 2039
87 C 15505 1122933 3028 6007
88 G 4789 2561 1127774 2713
89 T 3268 4651 5361 1158477
92 Full breakdown of errors by cycle and nucleotide
93 Cycle: Read As: Really:
102 2 C 1165 21533 73 159
107 3 C 1037 30720 97 230
112 4 C 894 31174 102 178
122 6 C 1079 31888 103 209
142 10 C 540 31637 69 166
147 11 C 493 31165 72 179
152 12 C 394 31650 70 132
157 13 C 313 30538 55 144
162 14 C 275 30682 60 137
167 15 C 329 31087 63 152
172 16 C 270 30913 66 105
177 17 C 343 30336 88 174
182 18 C 309 31234 66 152
184 18 T 62 112 130 31866
187 19 C 541 30946 69 151
192 20 C 359 30888 87 134
194 20 T 63 105 120 31928
197 21 C 222 31121 66 122
199 21 T 78 129 142 31839
202 22 C 244 31011 64 123
207 23 C 352 30770 65 158
209 23 T 75 118 133 32116
212 24 C 235 31056 76 160
214 24 T 78 122 163 32308
217 25 C 254 30607 69 160
219 25 T 72 117 141 32139
222 26 C 340 30453 70 150
224 26 T 81 110 125 32140
227 27 C 294 30725 74 158
229 27 T 85 122 122 32160
232 28 C 257 30705 79 126
234 28 T 96 138 181 32093
237 29 C 259 30448 79 121
239 29 T 102 142 161 32079
242 30 C 223 30650 87 155
243 30 G 123 103 30269 92
244 30 T 86 148 218 32020
247 31 C 221 30503 94 161
248 31 G 143 107 30476 107
249 31 T 117 177 224 31844
252 32 C 215 30236 94 164
253 32 G 150 118 30292 112
254 32 T 112 184 179 31893
256 33 A 31353 150 134 86
257 33 C 272 30280 118 191
258 33 G 215 120 30432 129
259 33 T 156 220 281 31821
261 34 A 31795 188 148 94
262 34 C 274 30016 127 157
263 34 G 260 217 30039 138
264 34 T 195 298 460 31499
266 35 A 31483 201 179 130
267 35 C 309 29892 111 202
268 35 G 217 223 30127 195
269 35 T 175 273 305 31808
271 36 A 31091 189 181 140
272 36 C 396 30610 116 226
273 36 G 265 230 29924 219
274 36 T 205 251 268 31481
276 37 A 31592 224 219 140
277 37 C 404 30044 197 242
278 37 G 316 249 29822 222
279 37 T 234 287 344 31332
281 Error rate relative to reference by cycle/nucleotide
282 Cycle: Read As: Really:
283 A prp C prp G prp T prp
284 @1 N 0.002 0.001 0.002 0.002
285 @1 A 0.989 0.002 0.004 0.003
286 @1 C 0.005 0.990 0.001 0.003
287 @1 G 0.003 0.002 0.990 0.001
288 @1 T 0.002 0.005 0.003 0.991
289 @2 N 0.001 0.001 0.001 0.001
290 @2 A 0.969 0.002 0.003 0.002
291 @2 C 0.027 0.993 0.002 0.006
292 @2 G 0.003 0.002 0.991 0.002
293 @2 T 0.001 0.003 0.003 0.989
294 @3 N 0.000 0.001 0.001 0.000
295 @3 A 0.965 0.001 0.003 0.002
296 @3 C 0.030 0.994 0.003 0.007
297 @3 G 0.003 0.001 0.990 0.001
298 @3 T 0.002 0.003 0.003 0.989
299 @4 N 0.001 0.001 0.001 0.000
300 @4 A 0.965 0.001 0.002 0.002
301 @4 C 0.028 0.995 0.003 0.006
302 @4 G 0.004 0.001 0.992 0.001
303 @4 T 0.003 0.003 0.002 0.990
304 @5 N 0.001 0.001 0.001 0.001
305 @5 A 0.968 0.001 0.002 0.001
306 @5 C 0.026 0.995 0.002 0.007
307 @5 G 0.003 0.001 0.993 0.002
308 @5 T 0.002 0.002 0.002 0.990
309 @6 N 0.001 0.001 0.001 0.001
310 @6 A 0.964 0.001 0.003 0.001
311 @6 C 0.031 0.996 0.003 0.007
312 @6 G 0.003 0.001 0.992 0.001
313 @6 T 0.002 0.002 0.002 0.989
314 @7 N 0.001 0.001 0.000 0.001
315 @7 A 0.981 0.001 0.003 0.002
316 @7 C 0.013 0.994 0.002 0.005
317 @7 G 0.003 0.001 0.993 0.002
318 @7 T 0.002 0.003 0.002 0.991
319 @8 N 0.001 0.001 0.001 0.001
320 @8 A 0.983 0.001 0.003 0.001
321 @8 C 0.011 0.994 0.002 0.005
322 @8 G 0.004 0.001 0.993 0.002
323 @8 T 0.001 0.003 0.002 0.991
324 @9 N 0.001 0.001 0.001 0.001
325 @9 A 0.979 0.001 0.002 0.001
326 @9 C 0.015 0.995 0.002 0.007
327 @9 G 0.003 0.001 0.993 0.002
328 @9 T 0.002 0.002 0.002 0.990
329 @10 N 0.001 0.000 0.001 0.001
330 @10 A 0.980 0.001 0.002 0.002
331 @10 C 0.016 0.994 0.002 0.006
332 @10 G 0.003 0.001 0.992 0.001
333 @10 T 0.001 0.003 0.002 0.991
334 @11 N 0.001 0.001 0.001 0.001
335 @11 A 0.980 0.001 0.002 0.001
336 @11 C 0.015 0.995 0.002 0.006
337 @11 G 0.003 0.001 0.993 0.002
338 @11 T 0.002 0.003 0.002 0.990
339 @12 N 0.001 0.001 0.001 0.001
340 @12 A 0.982 0.001 0.002 0.001
341 @12 C 0.012 0.995 0.002 0.004
342 @12 G 0.003 0.001 0.992 0.001
343 @12 T 0.002 0.002 0.002 0.993
344 @13 N 0.001 0.001 0.001 0.001
345 @13 A 0.985 0.001 0.003 0.002
346 @13 C 0.009 0.994 0.002 0.004
347 @13 G 0.003 0.001 0.991 0.001
348 @13 T 0.002 0.002 0.004 0.992
349 @14 N 0.001 0.001 0.000 0.000
350 @14 A 0.986 0.001 0.002 0.001
351 @14 C 0.008 0.993 0.002 0.004
352 @14 G 0.003 0.001 0.992 0.001
353 @14 T 0.002 0.003 0.003 0.993
354 @15 N 0.001 0.001 0.001 0.001
355 @15 A 0.984 0.001 0.002 0.002
356 @15 C 0.010 0.994 0.002 0.005
357 @15 G 0.003 0.001 0.992 0.002
358 @15 T 0.002 0.003 0.003 0.991
359 @16 N 0.001 0.000 0.001 0.001
360 @16 A 0.986 0.002 0.002 0.001
361 @16 C 0.008 0.993 0.002 0.003
362 @16 G 0.003 0.001 0.990 0.001
363 @16 T 0.002 0.003 0.005 0.994
364 @17 N 0.001 0.001 0.001 0.000
365 @17 A 0.984 0.002 0.002 0.001
366 @17 C 0.010 0.993 0.003 0.005
367 @17 G 0.003 0.002 0.990 0.001
368 @17 T 0.002 0.003 0.004 0.992
369 @18 N 0.001 0.001 0.000 0.001
370 @18 A 0.985 0.001 0.003 0.001
371 @18 C 0.010 0.993 0.002 0.005
372 @18 G 0.003 0.001 0.990 0.001
373 @18 T 0.002 0.004 0.004 0.992
374 @19 N 0.001 0.001 0.000 0.001
375 @19 A 0.978 0.001 0.003 0.001
376 @19 C 0.017 0.994 0.002 0.005
377 @19 G 0.003 0.001 0.992 0.001
378 @19 T 0.002 0.003 0.003 0.992
379 @20 N 0.001 0.001 0.001 0.001
380 @20 A 0.983 0.001 0.002 0.001
381 @20 C 0.011 0.994 0.003 0.004
382 @20 G 0.003 0.001 0.990 0.002
383 @20 T 0.002 0.003 0.004 0.992
384 @21 N 0.001 0.001 0.001 0.000
385 @21 A 0.987 0.002 0.002 0.001
386 @21 C 0.007 0.991 0.002 0.004
387 @21 G 0.003 0.002 0.990 0.001
388 @21 T 0.002 0.004 0.005 0.993
389 @22 N 0.001 0.001 0.001 0.001
390 @22 A 0.986 0.002 0.002 0.001
391 @22 C 0.007 0.994 0.002 0.004
392 @22 G 0.004 0.001 0.991 0.002
393 @22 T 0.002 0.003 0.004 0.993
394 @23 N 0.001 0.001 0.001 0.001
395 @23 A 0.983 0.001 0.002 0.001
396 @23 C 0.011 0.992 0.002 0.005
397 @23 G 0.003 0.001 0.990 0.002
398 @23 T 0.002 0.004 0.004 0.991
399 @24 N 0.001 0.001 0.001 0.001
400 @24 A 0.986 0.002 0.002 0.001
401 @24 C 0.007 0.991 0.003 0.005
402 @24 G 0.003 0.002 0.989 0.002
403 @24 T 0.002 0.004 0.005 0.992
404 @25 N 0.001 0.001 0.001 0.001
405 @25 A 0.987 0.002 0.003 0.001
406 @25 C 0.008 0.992 0.002 0.005
407 @25 G 0.003 0.001 0.989 0.002
408 @25 T 0.002 0.004 0.005 0.991
409 @26 N 0.001 0.001 0.001 0.001
410 @26 A 0.983 0.002 0.002 0.001
411 @26 C 0.010 0.992 0.002 0.005
412 @26 G 0.003 0.001 0.990 0.002
413 @26 T 0.002 0.004 0.004 0.992
414 @27 N 0.002 0.002 0.002 0.002
415 @27 A 0.982 0.002 0.003 0.002
416 @27 C 0.009 0.991 0.002 0.005
417 @27 G 0.004 0.002 0.989 0.002
418 @27 T 0.003 0.004 0.004 0.989
419 @28 N 0.002 0.002 0.002 0.002
420 @28 A 0.983 0.002 0.003 0.001
421 @28 C 0.008 0.989 0.003 0.004
422 @28 G 0.004 0.002 0.987 0.002
423 @28 T 0.003 0.004 0.006 0.991
424 @29 N 0.003 0.003 0.002 0.003
425 @29 A 0.982 0.002 0.002 0.001
426 @29 C 0.008 0.988 0.003 0.004
427 @29 G 0.004 0.003 0.988 0.003
428 @29 T 0.003 0.005 0.005 0.989
429 @30 N 0.003 0.003 0.003 0.004
430 @30 A 0.983 0.003 0.002 0.001
431 @30 C 0.007 0.986 0.003 0.005
432 @30 G 0.004 0.003 0.985 0.003
433 @30 T 0.003 0.005 0.007 0.987
434 @31 N 0.004 0.004 0.003 0.004
435 @31 A 0.981 0.004 0.003 0.002
436 @31 C 0.007 0.983 0.003 0.005
437 @31 G 0.004 0.003 0.983 0.003
438 @31 T 0.004 0.006 0.007 0.986
439 @32 N 0.005 0.004 0.004 0.004
440 @32 A 0.981 0.004 0.002 0.002
441 @32 C 0.007 0.982 0.003 0.005
442 @32 G 0.005 0.004 0.984 0.003
443 @32 T 0.003 0.006 0.006 0.986
444 @33 N 0.006 0.005 0.005 0.006
445 @33 A 0.974 0.005 0.004 0.003
446 @33 C 0.008 0.979 0.004 0.006
447 @33 G 0.007 0.004 0.978 0.004
448 @33 T 0.005 0.007 0.009 0.982
449 @34 N 0.006 0.006 0.006 0.006
450 @34 A 0.972 0.006 0.005 0.003
451 @34 C 0.008 0.972 0.004 0.005
452 @34 G 0.008 0.007 0.971 0.004
453 @34 T 0.006 0.010 0.015 0.982
454 @35 N 0.006 0.006 0.006 0.007
455 @35 A 0.972 0.007 0.006 0.004
456 @35 C 0.010 0.971 0.004 0.006
457 @35 G 0.007 0.007 0.975 0.006
458 @35 T 0.005 0.009 0.010 0.977
459 @36 N 0.006 0.007 0.007 0.007
460 @36 A 0.967 0.006 0.006 0.004
461 @36 C 0.012 0.972 0.004 0.007
462 @36 G 0.008 0.007 0.975 0.007
463 @36 T 0.006 0.008 0.009 0.975
464 @37 N 0.006 0.006 0.005 0.007
465 @37 A 0.965 0.007 0.007 0.004
466 @37 C 0.012 0.969 0.006 0.008
467 @37 G 0.010 0.008 0.970 0.007
468 @37 T 0.007 0.009 0.011 0.974
470 Information Content By Cycle
471 Bases this cycle Bases so far
472 Cycle: Equiv info: Align: Total: Equiv info: Align: Total:
473 ~1 121135.20 126433 221956 121135.20 126433 221956
474 ~2 118981.66 126544 220204 240116.88 252977 442160
475 ~3 118709.06 126574 219216 358825.94 379551 661376
476 ~4 119387.88 126568 218313 478213.81 506119 879689
477 ~5 119571.18 126556 217983 597785.00 632675 1097672
478 ~6 119189.21 126571 217603 716974.19 759246 1315275
479 ~7 120874.08 126565 217439 837848.25 885811 1532714
480 ~8 121116.88 126571 217155 958965.12 1012382 1749869
481 ~9 120779.02 126568 216903 1079744.12 1138950 1966772
482 ~10 120721.09 126562 217060 1200465.25 1265512 2183832
483 ~11 120762.78 126565 217584 1321228.00 1392077 2401416
484 ~12 121319.63 126565 217610 1442547.62 1518642 2619026
485 ~13 121292.62 126562 217564 1563840.25 1645204 2836590
486 ~14 121490.08 126579 217562 1685330.38 1771783 3054152
487 ~15 121105.89 126540 217267 1806436.25 1898323 3271419
488 ~16 121309.71 126576 216955 1927746.00 2024899 3488374
489 ~17 120892.80 126585 216878 2048638.75 2151484 3705252
490 ~18 120964.48 126576 216864 2169603.25 2278060 3922116
491 ~19 120717.50 126574 216842 2290320.75 2404634 4138958
492 ~20 120936.81 126564 216644 2411257.50 2531198 4355602
493 ~21 121079.27 126564 216639 2532336.75 2657762 4572241
494 ~22 121315.66 126561 216490 2653652.50 2784323 4788731
495 ~23 120625.85 126488 216289 2774278.25 2910811 5005020
496 ~24 120734.09 126547 216482 2895012.25 3037358 5221502
497 ~25 120758.35 126540 216381 3015770.50 3163898 5437883
498 ~26 120670.38 126527 216482 3136441.00 3290425 5654365
499 ~27 120291.03 126419 216156 3256732.00 3416844 5870521
500 ~28 120022.73 126417 216128 3376754.75 3543261 6086649
501 ~29 119949.20 126315 215933 3496704.00 3669576 6302582
502 ~30 119421.66 126224 215800 3616125.75 3795800 6518382
503 ~31 118718.27 126140 215620 3734844.00 3921940 6734002
504 ~32 118778.77 126087 215509 3853622.75 4048027 6949511
505 ~33 116782.13 125958 215248 3970405.00 4173985 7164759
506 ~34 115034.91 125905 215123 4085440.00 4299890 7379882
507 ~35 114996.52 125830 214997 4200436.50 4425720 7594879
508 ~36 114382.40 125792 214951 4314819.00 4551512 7809830
509 ~37 113076.75 125868 215037 4427896.00 4677380 8024867
511 The 30 most common words with 2 blanks or less:
512 Ranking Occurrences Words
513 1 411 GTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGT
514 2 382 TGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTG
515 3 324 TGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTG
516 4 271 GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGT
517 5 239 GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGT
518 6 234 AACACAACACAACACAACACAACACAACACAACACAA
519 7 214 ACAACACAACACAACACAACACAACACAACACAACAC
520 8 209 CACAACACAACACAACACAACACAACACAACACAACA
521 9 206 AAGACAAGACAAGACAAGACAAGACAAGACAAGACAA
522 10 203 AGACAAGACAAGACAAGACAAGACAAGACAAGACAAG CAAGACAAGACAAGACAAGACAAGACAAGACAAGACA
523 11 201 CAACACAACACAACACAACACAACACAACACAACACA
524 12 200 ACACAACACAACACAACACAACACAACACAACACAAC
525 13 170 ACAAGACAAGACAAGACAAGACAAGACAAGACAAGAC
526 14 152 TGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG
527 15 149 AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG
528 16 142 AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG
529 17 135 CTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCT
530 18 118 AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA
531 19 117 GACAAGACAAGACAAGACAAGACAAGACAAGACAAGA
532 20 103 TTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT
533 21 102 GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA
534 22 100 GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA
535 23 80 TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT TTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTT
536 24 76 TCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTC
537 25 53 CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT
538 26 47 CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT
539 27 42 TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC
540 28 37 TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC
541 29 36 GTCATGTCTTGTCATGTCTTGTCATGTCTTGTCATGT
542 30 35 CATGTCTTGTCATGTCTTGTCATGTCTTGTCATGTCT
544 The 30 most common blank patterns (N=any nonblank character)
545 Ranking Occurrences Words
546 1 209206 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
547 2 2010 N....................................
548 3 1020 NN...................................
549 4 727 NNN..................................
550 5 472 NNNN.................................
551 6 441 .....................................
552 7 422 NNNNN................................
553 8 376 .NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
554 9 206 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.......
555 10 191 .NNNNNNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNN
556 11 181 NNNNNNNN..NNNNN......................
557 12 180 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.N
558 13 162 NNNNNNNNNNNNNNNNNNNNNNNNNNNN.........
559 14 160 N.NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN......
560 15 158 NNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNNN
561 16 157 NNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNN
562 17 153 NNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNNN
563 18 152 NNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNN
564 19 150 NNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN....
565 20 148 NNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNN
566 21 146 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNN
567 22 145 NNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNN
568 23 144 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.
569 24 143 NNNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNNNNN
570 25 142 NNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
571 26 141 NNNNNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNN
572 27 140 NNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNNNN
573 28 139 NNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNN
574 29 137 NNNNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNN
575 30 136 NNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNN
577 Log likelihood scores
578 Cycle: Read As: Really:
581 >1 A 225 -289 -256 -282
582 >1 C -208 189 -282 -252
583 >1 G -237 -273 214 -296
584 >1 T -246 -229 -243 191
586 >2 A 233 -306 -262 -286
587 >2 C -127 118 -249 -215
588 >2 G -247 -296 220 -276
589 >2 T -267 -262 -243 208
591 >3 A 228 -288 -265 -278
592 >3 C -147 135 -251 -214
593 >3 G -242 -304 219 -277
594 >3 T -263 -252 -258 209
596 >4 A 230 -299 -266 -275
597 >4 C -154 142 -249 -225
598 >4 G -246 -306 224 -287
599 >4 T -257 -253 -265 210
601 >5 A 236 -298 -267 -293
602 >5 C -150 138 -258 -216
603 >5 G -244 -303 220 -275
604 >5 T -267 -267 -272 221
606 >6 A 235 -305 -260 -299
607 >6 C -147 136 -250 -219
608 >6 G -249 -304 227 -296
609 >6 T -272 -266 -267 220
611 >7 A 227 -286 -263 -279
612 >7 C -184 166 -277 -224
613 >7 G -245 -300 219 -273
614 >7 T -268 -262 -263 216
616 >8 A 226 -287 -256 -288
617 >8 C -191 170 -267 -228
618 >8 G -241 -285 213 -270
619 >8 T -286 -263 -273 225
621 >9 A 229 -284 -262 -290
622 >9 C -183 162 -267 -218
623 >9 G -243 -305 220 -276
624 >9 T -277 -267 -272 224
625 >10 N -43 -66 -48 -35
626 >10 A 228 -287 -264 -280
627 >10 C -177 161 -267 -228
628 >10 G -251 -299 227 -291
629 >10 T -285 -254 -264 218
630 >11 N -55 -38 -46 -52
631 >11 A 234 -296 -267 -289
632 >11 C -180 162 -264 -224
633 >11 G -249 -308 222 -271
634 >11 T -277 -256 -261 216
635 >12 N -52 -49 -38 -52
636 >12 A 234 -297 -262 -294
637 >12 C -190 172 -266 -238
638 >12 G -248 -299 224 -285
639 >12 T -274 -262 -261 217
640 >13 N -48 -43 -45 -54
641 >13 A 227 -288 -261 -282
642 >13 C -199 177 -275 -233
643 >13 G -252 -295 226 -286
644 >13 T -270 -262 -242 208
645 >14 N -44 -34 -54 -58
646 >14 A 235 -286 -267 -300
647 >14 C -205 181 -271 -235
648 >14 G -244 -293 222 -291
649 >14 T -271 -250 -251 208
650 >15 N -49 -47 -42 -51
651 >15 A 229 -287 -270 -275
652 >15 C -197 175 -269 -231
653 >15 G -242 -301 219 -279
654 >15 T -265 -258 -252 210
655 >16 N -46 -60 -40 -43
656 >16 A 230 -279 -265 -295
657 >16 C -206 184 -267 -247
658 >16 G -248 -281 221 -288
659 >16 T -267 -251 -233 200
660 >17 N -38 -38 -42 -77
661 >17 A 229 -284 -265 -285
662 >17 C -195 170 -254 -224
663 >17 G -252 -278 222 -288
664 >17 T -272 -256 -244 208
665 >18 N -29 -53 -64 -46
666 >18 A 224 -284 -255 -284
667 >18 C -200 177 -268 -231
668 >18 G -252 -283 223 -287
669 >18 T -271 -245 -239 202
670 >19 N -30 -48 -79 -39
671 >19 A 232 -301 -258 -292
672 >19 C -176 160 -266 -232
673 >19 G -252 -314 228 -283
674 >19 T -274 -249 -259 211
675 >20 N -36 -49 -58 -47
676 >20 A 232 -293 -265 -288
677 >20 C -193 172 -255 -236
678 >20 G -246 -300 221 -277
679 >20 T -270 -248 -242 204
680 >21 N -47 -41 -41 -62
681 >21 A 226 -267 -267 -291
682 >21 C -214 188 -267 -241
683 >21 G -247 -272 217 -282
684 >21 T -261 -239 -235 196
685 >22 N -43 -49 -41 -57
686 >22 A 232 -278 -271 -294
687 >22 C -210 185 -269 -240
688 >22 G -241 -286 216 -279
689 >22 T -261 -256 -238 203
690 >23 N -54 -45 -50 -40
691 >23 A 228 -284 -264 -283
692 >23 C -194 172 -268 -229
693 >23 G -252 -282 220 -275
694 >23 T -263 -243 -238 199
695 >24 N -43 -50 -43 -54
696 >24 A 228 -272 -266 -296
697 >24 C -212 181 -261 -229
698 >24 G -247 -265 213 -273
699 >24 T -262 -242 -229 194
700 >25 N -51 -49 -61 -31
701 >25 A 219 -273 -251 -282
702 >25 C -208 180 -265 -228
703 >25 G -253 -283 219 -269
704 >25 T -265 -244 -236 198
705 >26 N -53 -40 -40 -57
706 >26 A 231 -282 -265 -296
707 >26 C -195 173 -264 -231
708 >26 G -243 -284 213 -267
709 >26 T -260 -246 -241 200
710 >27 N -43 -57 -40 -49
711 >27 A 222 -281 -256 -275
712 >27 C -202 176 -262 -229
713 >27 G -237 -274 207 -261
714 >27 T -258 -242 -242 199
715 >28 N -34 -56 -55 -46
716 >28 A 220 -265 -255 -289
717 >28 C -208 182 -259 -239
718 >28 G -237 -261 203 -257
719 >28 T -252 -237 -225 188
720 >29 N -46 -47 -54 -42
721 >29 A 225 -264 -271 -285
722 >29 C -207 182 -259 -240
723 >29 G -234 -256 199 -253
724 >29 T -250 -235 -230 189
725 >30 N -46 -52 -53 -38
726 >30 A 215 -249 -262 -283
727 >30 C -214 181 -255 -230
728 >30 G -239 -247 197 -252
729 >30 T -257 -233 -217 185
730 >31 N -41 -50 -60 -39
731 >31 A 208 -245 -252 -278
732 >31 C -214 180 -251 -228
733 >31 G -233 -245 193 -245
734 >31 T -244 -225 -215 178
735 >32 N -44 -48 -50 -46
736 >32 A 210 -240 -262 -281
737 >32 C -215 180 -251 -227
738 >32 G -230 -241 190 -243
739 >32 T -245 -224 -225 182
740 >33 N -42 -56 -51 -41
741 >33 A 192 -232 -237 -256
742 >33 C -205 171 -241 -220
743 >33 G -215 -240 181 -237
744 >33 T -231 -216 -205 168
745 >34 N -46 -50 -50 -43
746 >34 A 186 -223 -233 -253
747 >34 C -204 173 -237 -228
748 >34 G -206 -214 168 -234
749 >34 T -221 -203 -184 151
750 >35 N -49 -48 -50 -41
751 >35 A 179 -219 -224 -238
752 >35 C -199 168 -243 -217
753 >35 G -214 -213 167 -219
754 >35 T -226 -207 -202 162
755 >36 N -48 -47 -48 -46
756 >36 A 178 -222 -223 -235
757 >36 C -189 161 -243 -213
758 >36 G -205 -212 162 -214
759 >36 T -219 -210 -207 163
760 >37 N -50 -48 -56 -36
761 >37 A 173 -215 -216 -235
762 >37 C -187 155 -219 -210
763 >37 G -198 -208 157 -213
764 >37 T -213 -204 -196 155
766 Cumulative errors by cycle
768 !1 125330 126433 126433 126433 126433
769 !2 123478 126485 126544 126544 126544
770 !3 121621 126432 126574 126574 126574
771 !4 119966 126327 126568 126568 126568
772 !5 118395 126151 126556 126556 126556
773 !6 116747 125952 126571 126571 126571
774 !7 115677 125775 126565 126565 126565
775 !8 114666 125627 126571 126571 126571
776 !9 113563 125445 126568 126568 126568
777 !10 112449 125247 126562 126562 126562
778 !11 111387 125030 126565 126565 126565
779 !12 110462 124835 126565 126565 126565
780 !13 109545 124634 126562 126562 126562
781 !14 108713 124442 126579 126579 126579
782 !15 107751 124171 126540 126540 126540
783 !16 106931 123971 126576 126576 126576
784 !17 106010 123696 126585 126585 126585
785 !18 105106 123393 126576 126576 126576
786 !19 104133 123046 126574 126574 126574
787 !20 103248 122711 126564 126564 126564
788 !21 102436 122390 126564 126564 126564
789 !22 101656 122077 126561 126561 126561
790 !23 100677 121658 126488 126488 126488
791 !24 99884 121345 126547 126547 126547
792 !25 99045 120962 126540 126540 126540
793 !26 98191 120538 126527 126527 126527
794 !27 97267 119962 126419 126419 126419
795 !28 96397 119475 126417 126417 126417
796 !29 95473 118877 126315 126315 126315
797 !30 94545 118189 126224 126224 126224
798 !31 93562 117440 126140 126140 126140
799 !32 92682 116663 126087 126087 126087
800 !33 91764 115889 125385 125958 125958
801 !34 90677 115109 124668 125814 125905
802 !35 89710 114249 123991 125563 125801
803 !36 88756 113426 123311 125289 125699
804 !37 87838 112643 122629 125025 125655