alg/nway_paths.cpp

   1 //  This file is part of the Mussa source distribution.
   2 //  http://mussa.caltech.edu/
   3 //  Contact author: Tristan  De Buysscher, tristan@caltech.edu
   4
   5 // This program and all associated source code files are Copyright (C) 2005
   6 // the California Institute of Technology, Pasadena, CA, 91125 USA.  It is
   7 // under the GNU Public License; please see the included LICENSE.txt
   8 // file for more information, or contact Tristan directly.
   9
  10
  11 //                        ----------------------------------------
  12 //                         ---------- mussa_nway.cc  -----------
  13 //                        ----------------------------------------
  14
  15 #include <boost/filesystem/fstream.hpp>
  16 namespace fs = boost::filesystem;
  17
  18 #include "alg/mussa_callback.hpp"
  19 #include "alg/nway_paths.hpp"
  20 #include "alg/conserved_path.hpp"
  21 #include "mussa_exceptions.hpp"
  22
  23 #include <iostream>
  24 #include <stdexcept>
  25
  26 using namespace std;
  27
  28 NwayPaths::NwayPaths()
  29 {
  30 }
  31
  32 NwayPaths::NwayPaths(const NwayPaths &o)
  33   : pathz(o.pathz),
  34     refined_pathz(o.refined_pathz),
  35     threshold(o.threshold),
  36     win_size(o.win_size),
  37     soft_thres(o.soft_thres),
  38     ent_thres(o.ent_thres),
  39     c_sequences(o.c_sequences)
  40 {
  41 }
  42
  43 void NwayPaths::clear()
  44 {
  45   c_sequences.clear();
  46   pathz.clear();
  47   refined_pathz.clear();
  48 }
  49
  50 void
  51 NwayPaths::setup(int w, int t)
  52 {
  53   threshold = t;
  54   soft_thres = threshold;
  55   win_size = w;
  56   clear();
  57
  58   //cout << "nway: thres = " << threshold
  59   //     << ", soft threo = " << soft_thres << endl;
  60 }
  61
  62 void
  63 NwayPaths::set_soft_threshold(int sft_thres)
  64 {
  65   soft_thres = sft_thres;
  66 }
  67
  68 int NwayPaths::get_soft_threshold() const
  69 {
  70   return soft_thres;
  71 }
  72
  73 int NwayPaths::get_threshold() const
  74 {
  75   return threshold;
  76 }
  77
  78 int NwayPaths::get_window() const
  79 {
  80   return win_size;
  81 }
  82
  83 // dumbly goes thru and combines path windows exactly adjacent (ie + 1 index)
  84 // doesn't deal with interleaved adjacency
  85 void
  86 NwayPaths::simple_refine()
  87 {
  88   // ext_path remembers the first window set in an extending path
  89   ConservedPath ext_path, new_path;
  90   list<ConservedPath>::iterator cur_path, next_path;
  91   list<ConservedPath>::iterator pathz_i;
  92   int win_ext_len = 0;
  93   bool extending, end = false;
  94
  95   refined_pathz.clear();
  96
  97   //cout << "path number is: " << pathz.size() << endl;
  98   pathz_i = pathz.begin();
  99   int path_count = 0;
 100
 101   // only try to extend when pathz isn't empty.
 102   if (pathz_i != pathz.end())
 103   {
 104     ext_path = *pathz_i;
 105     ++path_count;
 106
 107     while(pathz_i != pathz.end())
 108     {
 109       // keep track of current path and advance to next path
 110       cur_path = pathz_i;
 111       ++pathz_i;
 112       ++path_count;
 113
 114       if (pathz_i == pathz.end()) {
 115         end = true;
 116         extending = false;
 117       } else {
 118         next_path = pathz_i;
 119         // if node for each seq is equal to the next node+1 then for all
 120         // sequences then we are extending
 121         extending = cur_path->nextTo(*next_path);
 122       }
 123
 124       if (extending)
 125       {
 126         win_ext_len++;
 127       }
 128       else
 129       {
 130         // add the extend window length as first element and add as refined
 131         // now that we have the path to extend save it
 132         new_path = ext_path;
 133         new_path.extend(win_ext_len);
 134         refined_pathz.push_back(new_path);
 135         if (not end) {
 136           // reset stuff
 137           win_ext_len = 0;
 138           ext_path = *next_path;
 139         }
 140       }
 141       if ((path_count % 100) == 0)
 142         emit progress("refine", path_count-1, pathz.size());
 143     }
 144   }
 145   // this mysterious call tells the dialog box that we're actually done
 146   emit progress("refine", pathz.size(), pathz.size());
 147   //cout << "r_path number is: " << refined_pathz.size() << endl;
 148 }
 149
 150 void
 151 NwayPaths::add_path(int threshold, vector<int>& loaded_path)
 152 {
 153   pathz.push_back(ConservedPath(threshold, 0.0, loaded_path));
 154 }
 155
 156 void
 157 NwayPaths::add_path(ConservedPath loaded_path)
 158 {
 159   pathz.push_back(loaded_path);
 160 }
 161
 162
 163 void
 164 NwayPaths::save(fs::path save_file_path)
 165 {
 166   fs::fstream save_file;
 167   list<ConservedPath >::iterator path_i, paths_end;
 168
 169   save_file.open(save_file_path, ios::out);
 170
 171   save_file << "<Mussa type=flp seq_count=" << sequence_count();
 172   save_file << " win=" << win_size;
 173   // add a function para new_thres defaults to -1 to later deal with
 174   // reanalysis with higher thres - if statement whether to record base thres
 175   // or new thres (ie if -1, then base)
 176   save_file << " thres=" << threshold << " >\n";
 177
 178   path_i = refined_pathz.begin();
 179   paths_end = refined_pathz.end();
 180   //path_i = pathz.begin();
 181   //paths_end = pathz.end();
 182   while (path_i != paths_end)
 183   {
 184     ConservedPath& a_path = *path_i;
 185     //cout << a_path.size() << endl;
 186     //first entry is the window length of the windows in the path
 187     save_file << a_path.window_size << ":";
 188     for(size_t i = 0; i != sequence_count(); ++i)
 189     {
 190       save_file << a_path[i];
 191       if (i != sequence_count())
 192         save_file << ",";
 193     }
 194     save_file << endl;
 195     ++path_i;
 196   }
 197
 198   save_file << "</Mussa>\n";
 199   save_file.close();
 200 }
 201
 202
 203 size_t
 204 NwayPaths::sequence_count()
 205 {
 206   if (refined_pathz.begin() == refined_pathz.end() )
 207     return 0;
 208   else
 209     return refined_pathz.begin()->size();
 210 }
 211
 212
 213 void
 214 NwayPaths::load(fs::path load_file_path)
 215 {
 216   fs::fstream load_file;
 217   string file_data_line, header_data, data, path_node, path_width;
 218   int space_split_i, equal_split_i, comma_split_i, colon_split_i;
 219   vector<int> loaded_path;
 220
 221   load_file.open(load_file_path, ios::in);
 222
 223   if (!load_file)
 224   {
 225     throw mussa_load_error("Sequence File: " + load_file_path.string() + " not found");
 226   }
 227   else
 228   {
 229     // get header data
 230     // grab mussa tag - discard for now...maybe check in future...
 231     getline(load_file,file_data_line);
 232     space_split_i = file_data_line.find(" ");
 233     file_data_line = file_data_line.substr(space_split_i+1);
 234     // grab type tag - need in future to distinguish between flp and vlp paths
 235     space_split_i = file_data_line.find(" ");
 236     file_data_line = file_data_line.substr(space_split_i+1);
 237     // get species/seq number
 238     space_split_i = file_data_line.find(" ");
 239     header_data = file_data_line.substr(0,space_split_i);
 240     equal_split_i = header_data.find("=");
 241     data = file_data_line.substr(equal_split_i+1);
 242     unsigned int species_num = atoi (data.c_str());
 243     file_data_line = file_data_line.substr(space_split_i+1);
 244     // get window size
 245     space_split_i = file_data_line.find(" ");
 246     header_data = file_data_line.substr(0,space_split_i);
 247     equal_split_i = header_data.find("=");
 248     data = file_data_line.substr(equal_split_i+1);
 249     win_size = atoi (data.c_str());
 250     file_data_line = file_data_line.substr(space_split_i+1);
 251     // get threshold size
 252     space_split_i = file_data_line.find(" ");
 253     header_data = file_data_line.substr(0,space_split_i);
 254     equal_split_i = header_data.find("=");
 255     data = file_data_line.substr(equal_split_i+1);
 256     threshold = atoi (data.c_str());
 257     file_data_line = file_data_line.substr(space_split_i+1);
 258
 259     //cout << "seq_num=" << species_num << " win=" << win_size;
 260     //cout << " thres=" << threshold << endl;
 261
 262     // clear out the current data
 263     refined_pathz.clear();
 264
 265     int temp;
 266
 267     getline(load_file,file_data_line);
 268     while ( (!load_file.eof()) && (file_data_line != "</Mussa>") )
 269     {
 270       if (file_data_line != "")
 271       {
 272         loaded_path.clear();
 273         colon_split_i = file_data_line.find(":");
 274         // whats our window size?
 275         path_width = file_data_line.substr(0,colon_split_i);
 276         file_data_line = file_data_line.substr(colon_split_i+1);
 277         for(size_t i = 0; i < species_num; i++)
 278         {
 279           comma_split_i = file_data_line.find(",");
 280           path_node = file_data_line.substr(0, comma_split_i);
 281           temp = atoi (path_node.c_str());
 282           loaded_path.push_back(temp);
 283           file_data_line = file_data_line.substr(comma_split_i+1);
 284         }
 285         assert (loaded_path.size() == species_num );
 286         refined_pathz.push_back(ConservedPath(atoi(path_width.c_str()),
 287                                                    threshold,
 288                                                    loaded_path));
 289       }
 290       getline(load_file,file_data_line);
 291     }
 292     load_file.close();
 293   }
 294 }
 295
 296
 297 void
 298 NwayPaths::path_search(vector<vector<FLPs> > all_comparisons, ConservedPath path, size_t depth)
 299 {
 300   list<int> new_nodes, trans_check_nodes;
 301   list<int>::iterator new_nodes_i, new_nodes_end;
 302   bool trans_check_good;
 303
 304   new_nodes = all_comparisons[depth - 1][depth].match_locations(path[depth-1]);
 305   new_nodes_i = new_nodes.begin();
 306   new_nodes_end = new_nodes.end();
 307   while(new_nodes_i != new_nodes_end)
 308   {
 309     //cout << "    * species " << depth << " node: " << *new_nodes_i << endl;
 310     // check transitivity with previous nodes in path
 311     trans_check_good = true;
 312     for(size_t i = 0; i < depth - 1; i++)
 313     {
 314       trans_check_nodes = all_comparisons[i][depth].match_locations(path[i]);
 315       if ( (trans_check_nodes.end() == find(trans_check_nodes.begin(),
 316                                             trans_check_nodes.end(),
 317                                             *new_nodes_i) ) &&
 318            (trans_check_nodes.end() == find(trans_check_nodes.begin(),
 319                                            trans_check_nodes.end(),
 320                                            *new_nodes_i * -1) ) )
 321         trans_check_good = false;
 322     }
 323
 324     if (trans_check_good)
 325     {
 326       // this makes sure path nodes are recorded with RC status relative to
 327       // the base species
 328       if ( path[depth-1] >= 0)
 329         path.push_back(*new_nodes_i);
 330       else
 331         path.push_back(*new_nodes_i * -1);
 332
 333       if (depth < all_comparisons.size() - 1)
 334         path_search(all_comparisons, path, depth + 1);
 335       else
 336         pathz.push_back(path);
 337       path.pop_back();
 338     }
 339     ++new_nodes_i;
 340   }
 341 }
 342 /* use this if I ever get the friggin seqcomp match lists to sort...
 343       if (binary_search(trans_check_nodes.begin(), trans_check_nodes.end(),
 344                         *new_nodes_i))
 345 */
 346
 347 void
 348 NwayPaths::find_paths_r(vector<vector<FLPs> > all_comparisons)
 349 {
 350   ConservedPath path;
 351   int win_i, window_num;
 352   list<int> new_nodes;
 353   list<int>::iterator new_nodes_i, new_nodes_end;
 354
 355   pathz.clear();
 356   window_num = all_comparisons[0][1].size();
 357   // loop thru all windows in first species
 358   for (win_i = 0; win_i < window_num; win_i++)
 359   {
 360     path.clear();
 361     path.push_back(win_i);
 362     new_nodes = all_comparisons[0][1].match_locations(path[0]);
 363     new_nodes_i = new_nodes.begin();
 364     new_nodes_end = new_nodes.end();
 365     //if (new_nodes_i != new_nodes_end)
 366     //cout << "* species 0 node: " << win_i << endl;
 367     path.push_back(0);
 368     while(new_nodes_i != new_nodes_end)
 369     {
 370       //cout << "  * species 1 node: " << *new_nodes_i << endl;
 371       path[1] = *new_nodes_i;
 372       path_search(all_comparisons, path, 2);
 373       ++new_nodes_i;
 374     }
 375   }
 376 }
 377
 378
 379 void
 380 NwayPaths::save_old(fs::path save_file_path)
 381 {
 382   fs::fstream save_file;
 383   list<ConservedPath >::iterator path_i, paths_end;
 384   size_t i;
 385
 386   save_file.open(save_file_path, ios::app);
 387
 388   path_i = pathz.begin();
 389   paths_end = pathz.end();
 390   while(path_i != paths_end)
 391   {
 392     ConservedPath& a_path = *path_i;
 393     //cout << a_path.size() << endl;
 394     for(i = 0; i < path_i->size(); ++i)
 395       save_file << i << "," << a_path[i] << " ";
 396     save_file << endl;
 397     ++path_i;
 398   }
 399   save_file.close();
 400 }
 401
 402
 403 /*
 404 void
 405 NwayPaths::find_paths(vector<vector<FLPs> > all_comparisons)
 406 {
 407   int win_i, sp_i;
 408   vector<list<list<int> > > path_src_tree;
 409   list<int> new_nodes;
 410   list<int>::iterator node_i, node_end;
 411   <list<list<int> > >::iterator branch_i, branch_end;
 412
 413   pathz.clear();
 414   path_src_tree.reserve(all_comparisons.size()- 1);
 415
 416
 417   // loop thru all windows in first species
 418   for (win_i = 0; win_i < window_num; win_i++)
 419   {
 420     // clear the path search tree
 421     for(i = 0; i < all_comparisons.size(); i++)
 422       path_src_tree[i].clear();
 423
 424     // top level kept empty even tho implicity has one entry of the first
 425     // species at this window - why bother, adds a little speed
 426
 427     // get connection list for first species, creating a list of nodes
 428     // of second species connected to the first species at this window
 429     new_nodes = all_comparisons[0][1];
 430     path_src_tree[1].push_back(new_nodes);
 431
 432     // loop thru rest of species for this window to see if any paths of matches
 433     // go across all species
 434     // if path search tree becomes empty, break out of loop, no reason to search further
 435     sp_i = 1;
 436     while ((sp_i < all_comparisons.size()) && (path tree not empty))
 437     {
 438       branch_i = path_src_tree[1].begin();
 439       branch_end = path_src_tree[1].end();
 440       while (branch_i != branch_end)
 441       {
 442         node_i = branch_i->begin();
 443         node_end = branch_i->end();
 444       }
 445
 446
 447       // loop over all current nodes
 448          // get connection list for each node
 449          // loop over each previous node in list
 450             // get those nodes connection list
 451             // intersect previous node connections with current
 452
 453       ++sp_i;
 454     }
 455
 456     // insert any of the paths found into the master list of paths
 457
 458     // add no paths if tmp_pathz is empty...
 459   }
 460 }
 461
 462 void NwayPaths::refine()
 463 {
 464 }
 465 */
 466
 467
 468 void NwayPaths::print(list<vector<int> >& dump_path)
 469 {
 470   list<vector<int> >::iterator pathz_i;
 471   vector<int>::iterator path_i;
 472
 473   cout << "printing list of lists\n";
 474   for (pathz_i = dump_path.begin(); pathz_i != dump_path.end(); ++pathz_i)
 475   {
 476     for (path_i = pathz_i->begin(); path_i != pathz_i->end(); ++path_i)
 477       cout << *path_i << " ";
 478     cout << endl;
 479   }
 480 }