1 #include <boost/test/auto_unit_test.hpp>
2 #include <boost/filesystem/path.hpp>
3 #include <boost/filesystem/operations.hpp>
4 namespace fs = boost::filesystem;
5 #include <boost/assign/list_of.hpp>
6 #include <boost/assign/list_inserter.hpp>
7 #include <boost/assign.hpp>
8 namespace assign = boost::assign;
14 #include "alg/mussa.hpp"
15 #include "mussa_exceptions.hpp"
19 //! can we initialize a mussa object?
20 BOOST_AUTO_TEST_CASE( mussa_simple )
23 BOOST_CHECK_EQUAL(m.empty(), true);
24 BOOST_CHECK_EQUAL(m.get_name(), "" );
25 BOOST_CHECK_EQUAL(m.get_window(), 0);
26 BOOST_CHECK_EQUAL(m.get_threshold(), 0);
27 BOOST_CHECK_EQUAL(m.get_analysis_mode(), Mussa::TransitiveNway);
28 m.set_name( "hello" );
29 BOOST_CHECK_EQUAL(m.get_name(), "hello" );
31 BOOST_CHECK_EQUAL(m.get_window(), 30);
33 BOOST_CHECK_EQUAL(m.get_threshold(), 21);
34 BOOST_CHECK_EQUAL(m.get_soft_threshold(), 21);
35 m.set_soft_threshold(19);
36 BOOST_CHECK_EQUAL(m.get_soft_threshold(), 21);
37 m.set_soft_threshold(35);
38 BOOST_CHECK_EQUAL(m.get_soft_threshold(), 30);
39 m.set_soft_threshold(25);
40 BOOST_CHECK_EQUAL(m.get_soft_threshold(), 25);
41 m.set_analysis_mode(Mussa::RadialNway);
42 BOOST_CHECK_EQUAL(m.get_analysis_mode(), Mussa::RadialNway);
43 // make sure our path is empty
44 BOOST_CHECK_EQUAL(m.get_analysis_path().string(), fs::path().string() );
47 BOOST_CHECK_EQUAL(m.get_name(), "" );
48 BOOST_CHECK_EQUAL(m.get_window(), 0);
49 BOOST_CHECK_EQUAL(m.get_threshold(), 0);
50 BOOST_CHECK_EQUAL(m.get_analysis_mode(), Mussa::TransitiveNway);
53 BOOST_AUTO_TEST_CASE ( mussa_title )
57 BOOST_CHECK_EQUAL( m.get_title(), "Unnamed");
60 BOOST_CHECK_EQUAL( m.get_title(), foo);
61 string foopath_name("/my/silly/path");
62 fs::path foopath(foopath_name);
63 m.set_analysis_path(foopath);
64 BOOST_CHECK_EQUAL( m.get_title().size(), 14);
67 BOOST_AUTO_TEST_CASE( mussa_analysis_name )
70 m.set_analysis_mode( Mussa::TransitiveNway );
71 BOOST_CHECK_EQUAL( m.get_analysis_mode_name(), "Transitive" );
72 m.set_analysis_mode( Mussa::RadialNway );
73 BOOST_CHECK_EQUAL( m.get_analysis_mode_name(), "Radial" );
74 m.set_analysis_mode( Mussa::EntropyNway );
75 BOOST_CHECK_EQUAL( m.get_analysis_mode_name(), "Entropy" );
76 m.set_analysis_mode( Mussa::RecursiveNway);
77 BOOST_CHECK_EQUAL( m.get_analysis_mode_name(), "[deprecated] Recursive" );
80 BOOST_AUTO_TEST_CASE( mussa_sequences )
82 std::string s0("AAAANNNN");
83 std::string s1("GGGGNNNN");
84 std::string s2("TTTTNNNN");
87 BOOST_CHECK_EQUAL(analysis.empty(), true);
88 analysis.append_sequence(s0);
89 analysis.append_sequence(s1);
90 analysis.append_sequence(s2);
92 BOOST_CHECK_EQUAL( analysis.empty(), false);
93 BOOST_CHECK_EQUAL( analysis.sequences().size(), 3 );
94 BOOST_CHECK_EQUAL( *(analysis.sequences()[0]), s0);
95 BOOST_CHECK_EQUAL( *(analysis.sequences()[1]), s1);
96 BOOST_CHECK_EQUAL( *(analysis.sequences()[2]), s2);
99 // for some reason we can call nway once safely but it
100 // somehow changed things and caused a segfault
101 // fixed by adding a return statement in trans_path_search
102 BOOST_AUTO_TEST_CASE ( empty_mussa_set_threshold )
105 m.set_soft_threshold(15);
108 m.set_soft_threshold(25);
112 BOOST_AUTO_TEST_CASE( mussa_load_mupa )
114 fs::path mupa_path(EXAMPLE_DIR, fs::native);
115 fs::path result_path = fs::initial_path() / "mck3test_w30_t20";
116 mupa_path /= "mck3test.mupa";
119 m1.load_mupa_file( mupa_path );
121 m1.save( result_path );
122 BOOST_CHECK_EQUAL( m1.empty(), false);
123 BOOST_CHECK_EQUAL( m1.get_name(), std::string("mck3test") );
124 BOOST_CHECK( m1.size() > 0 );
125 BOOST_CHECK_EQUAL( m1.get_analysis_path().string(), result_path.string());
128 m2.load( result_path );
129 BOOST_CHECK_EQUAL( m2.empty(), false);
130 BOOST_CHECK_EQUAL( m2.get_name(), result_path.leaf() );
131 BOOST_CHECK_EQUAL( m1.size(), m2.size() );
132 BOOST_CHECK_EQUAL( result_path.string(), m2.get_analysis_path().string() );
136 BOOST_CHECK_EQUAL( m2.empty(), true);
137 BOOST_CHECK_EQUAL( m2.is_dirty(), false );
138 BOOST_CHECK_EQUAL( m2.get_analysis_path().string(), fs::path().string());
141 BOOST_AUTO_TEST_CASE( mussa_load_full_path )
144 fs::path full_path(fs::path(EXAMPLE_DIR, fs::native) / "mck3test.mupa");
145 m1.load_mupa_file( full_path );
148 BOOST_CHECK( m1.size() > 0);
149 BOOST_CHECK_EQUAL( m1.get_window(), 30 );
150 BOOST_CHECK_EQUAL( m1.get_threshold(), 20);
151 BOOST_CHECK_EQUAL( m1.is_dirty(), true);
152 BOOST_CHECK_EQUAL( m1.get_analysis_path().string(), "");
155 // make sure we know that mupa files cannot be directories
156 BOOST_AUTO_TEST_CASE( mussa_mupa_is_file_not_directory )
158 fs::path curdir(".");
160 BOOST_CHECK_THROW(m1.load_mupa_file( curdir ), mussa_load_error );
163 BOOST_AUTO_TEST_CASE( mussa_load_analysis )
165 fs::path example_dir(EXAMPLE_DIR, fs::native);
167 m1.load_mupa_file( example_dir / "mck3test.mupa" );
171 fs::path analysis_path = fs::initial_path() / "mck3test_w30_t20";
172 m2.load( analysis_path );
174 BOOST_CHECK_EQUAL( m1.size(), m2.size() );
175 BOOST_CHECK_EQUAL( m1.get_window(), m2.get_window() );
176 BOOST_CHECK_EQUAL( m1.get_threshold(), m2.get_threshold() );
177 BOOST_CHECK_EQUAL( m2.get_analysis_path().string(), analysis_path.string());
180 BOOST_AUTO_TEST_CASE( mussa_load_motif )
182 string data = "AAGG 1.0 1.0 0.0\n"
186 istringstream test_istream(data);
189 m1.append_sequence("AAAAGGGGTTTT");
190 m1.append_sequence("GGGCCCCTTCCAATT");
191 m1.load_motifs(test_istream);
193 for (Mussa::vector_sequence_type::const_iterator seq_i = m1.sequences().begin();
194 seq_i != m1.sequences().end();
197 BOOST_CHECK( (*seq_i)->motifs().size() > 0 );
201 BOOST_AUTO_TEST_CASE( mussa_named_motif )
203 string data = "CCAATT cat 0.1 0.2 0.3\n";
204 istringstream test_istream(data);
207 m1.append_sequence("AAAAGGGGTTTT");
208 m1.append_sequence("GGGCCCCTTCCAATT");
209 m1.load_motifs(test_istream);
211 std::set<Sequence> motifs = m1.motifs();
212 BOOST_REQUIRE_EQUAL(motifs.size(), 1);
213 BOOST_CHECK_EQUAL(motifs.begin()->get_name(), "cat");
216 BOOST_AUTO_TEST_CASE( mussa_weirdly_spaced_named_motif )
218 string data = "CCAATT cat_meow123 0.1 0.2 0.3\n";
219 istringstream test_istream(data);
222 m1.append_sequence("AAAAGGGGTTTT");
223 m1.append_sequence("GGGCCCCTTCCAATT");
224 m1.load_motifs(test_istream);
226 std::set<Sequence> motifs = m1.motifs();
227 BOOST_REQUIRE_EQUAL(motifs.size(), 1);
228 BOOST_CHECK_EQUAL(motifs.begin()->get_name(), "cat_meow123");
230 BOOST_AUTO_TEST_CASE( mussa_add_motif )
232 vector<Sequence> motifs;
233 motifs.push_back("AAGG");
234 vector<Color> colors;
235 colors.push_back(Color(1.0, 0.0, 0.0));
238 m1.append_sequence("AAAAGGGGTTTT");
239 m1.append_sequence("GGGCCCCTTGGTT");
240 m1.set_motifs(motifs, colors);
241 int first_size = m1.motifs().size();
242 BOOST_CHECK_EQUAL( first_size, 1 );
243 BOOST_REQUIRE(first_size > 0);
244 BOOST_CHECK_EQUAL(*(m1.motifs().begin()), motifs.front());
245 // make sure that our sequences have the right number of motifs
246 BOOST_CHECK_EQUAL(m1.sequences()[0]->motifs().size(), 1);
247 BOOST_CHECK_EQUAL(m1.sequences()[1]->motifs().size(), 1); // because of rc
249 // verify that setting the motif clears the arrays
250 m1.set_motifs(motifs, colors);
251 BOOST_CHECK_EQUAL( first_size, m1.motifs().size() );
252 // make sure that our sequences have the right number of motifs
253 BOOST_CHECK_EQUAL(m1.sequences()[0]->motifs().size(), 1);
254 BOOST_CHECK_EQUAL(m1.sequences()[1]->motifs().size(), 1);
256 // add a different motif
258 motifs.push_back("CCTTGG");
259 BOOST_CHECK_EQUAL(motifs.size(), 1);
260 m1.set_motifs(motifs, colors);
261 BOOST_CHECK_EQUAL(m1.motifs().size(), 1);
262 BOOST_REQUIRE(m1.motifs().size() > 0);
263 BOOST_CHECK_EQUAL(*(m1.motifs().begin()), motifs.front());
264 BOOST_CHECK_EQUAL(m1.sequences()[0]->motifs().size(), 0);
265 BOOST_CHECK_EQUAL(m1.sequences()[1]->motifs().size(), 1);
267 // try a motif that doesn't exist
269 motifs.push_back("CCTTGG");
270 BOOST_CHECK_EQUAL(motifs.size(), 1);
271 m1.set_motifs(motifs, colors);
272 BOOST_CHECK_EQUAL(m1.motifs().size(), 1);
273 BOOST_CHECK_EQUAL(m1.sequences()[0]->motifs().size(), 0);
274 BOOST_CHECK_EQUAL(m1.sequences()[1]->motifs().size(), 1);
279 local_align_test(const Mussa::vector_sequence_type &seqs,
280 const list<ConservedPath::path_type>& result,
281 const list<vector<bool> >& reversed)
283 map<char, vector <char> > m;
284 assign::insert(m)('A', assign::list_of('A')('T') )
285 ('T', assign::list_of('T')('A') )
286 ('G', assign::list_of('G')('C') )
287 ('C', assign::list_of('C')('G') );
288 list<vector<bool> >::const_iterator rc_i = reversed.begin();
290 for(list<ConservedPath::path_type>::const_iterator base_i = result.begin();
291 base_i != result.end();
294 // since the reverse compliment flag is relative to the first sequence
295 // the first one should always be false
296 BOOST_CHECK_EQUAL( (*rc_i)[0], false );
297 const int first_path_basepair_index = (*base_i)[0];
298 const int second_path_basepair_index = (*base_i)[1];
299 const char first_basepair = (*seqs[0])[first_path_basepair_index];
300 const char second_basepair = (*seqs[1])[second_path_basepair_index];
301 // get our index into our reverse compliment map m
302 const int second_compliment_index = (*rc_i)[1];
303 // lookup the forward or reverse compliment depending on our rc flag
304 const char complimented_second = m[second_basepair][second_compliment_index];
306 BOOST_CHECK_EQUAL( first_basepair, complimented_second) ;
311 BOOST_AUTO_TEST_CASE( local_alignment )
313 string s0("GCGCATAT");
314 string s1("AAAAAAAT");
318 analysis.append_sequence(s0);
319 analysis.append_sequence(s1);
320 analysis.set_threshold(3);
321 analysis.set_window(4);
323 NwayPaths npath = analysis.paths();
324 list<ConservedPath::path_type> result;
325 list<vector<bool> > reversed;
326 list<ConservedPath>::iterator pathz_i = npath.pathz.begin();
328 list<ConservedPath> selected_paths;
329 selected_paths.push_back(*pathz_i);
330 analysis.createLocalAlignment(selected_paths.begin(),
331 selected_paths.end(),
335 local_align_test(analysis.sequences(), result, reversed);
340 selected_paths.clear();
341 selected_paths.push_back(*pathz_i);
342 analysis.createLocalAlignment(selected_paths.begin(),
343 selected_paths.end(),
346 local_align_test(analysis.sequences(), result, reversed);
351 BOOST_AUTO_TEST_CASE( subanalysis )
353 Sequence s1("AATGAAGATTTTAATGCTTTAATTTTGTTTTGTAAACTTCGAATTTCCAAAATTTGAAA");
354 Sequence s2("AGGAGCAAGTTCGCTTCATCGAGAATTTTTAATTTTTAGTCAAATTTTCCAATGTCTGA");
357 analysis.append_sequence(s1);
358 analysis.append_sequence(s2);
359 analysis.set_threshold(8);
360 analysis.set_window(8);
363 NwayPaths perfect_path = analysis.paths();
364 int perfect_match_count = perfect_path.pathz.size();
366 Sequence sub1 = s1.subseq(2, s1.size()-4);
367 Sequence sub2 = s2.subseq(2, s2.size()-4);
369 subanalysis.append_sequence(sub1);
370 subanalysis.append_sequence(sub2);
371 subanalysis.set_threshold(7);
372 subanalysis.set_window(8);
373 subanalysis.analyze();
374 NwayPaths one_mismatch_path = subanalysis.paths();
375 int one_mismatch_count = one_mismatch_path.pathz.size();
377 BOOST_CHECK( perfect_match_count < one_mismatch_count );
380 BOOST_AUTO_TEST_CASE( dirty_flag )
383 BOOST_CHECK_EQUAL(m.is_dirty(), false);
385 BOOST_CHECK_EQUAL(m.is_dirty(), true);
388 BOOST_CHECK_EQUAL(m.is_dirty(), true);
391 BOOST_CHECK_EQUAL(m.is_dirty(), true);
393 m.set_soft_threshold(1);
394 BOOST_CHECK_EQUAL(m.is_dirty(), false);
396 m.append_sequence("AAGGCCTT");
397 BOOST_CHECK_EQUAL(m.is_dirty(), true);