* Updated motif file format
* Updated UCSC Genome Broswer section slightly
-==============
-Mussagl Manual
-==============
+===================
+Mussagl Manual v1.0
+===================
---------------
Brandon W. King
---------------
---------------
Brandon W. King
---------------
-Last updated: Oct 27th, 2006
-
-Documentation for Mussagl v1.0
-
+Last updated: Oct 31st, 2006
.. Things to add
* New features / change log
.. Things to add
* New features / change log
.. ----------
.. INSERT CHANGE LOG HERE
.. ----------
.. INSERT CHANGE LOG HERE
- <motif> <red> <green> <blue>
+::
+
+ <motif> <optional name> <red> <green> <blue> <optional alpha>
+ AGTGAG "My First Motif" 0.333333 0.588235 1 1
+ ATGAT "2nd Motif" 1 0 0 1
you prefer to skip this section of the manual.
UCSC Genome Browser Method
you prefer to skip this section of the manual.
UCSC Genome Browser Method
---------------------------
+~~~~~~~~~~~~~~~~~~~~~~~~~~
There are many methods of retrieving DNA sequence, but for this
example we will retrieve SMN1 through the UCSC genome browser located
There are many methods of retrieving DNA sequence, but for this
example we will retrieve SMN1 through the UCSC genome browser located
:align: center
Step 1 - Find SMN1
:align: center
Step 1 - Find SMN1
The first step in finding SMN1 is to use the **Gene Sorter** menu
option which I have highlighted in orange below:
The first step in finding SMN1 is to use the **Gene Sorter** menu
option which I have highlighted in orange below:
Step 2 - Download CDS/UTR sequence for annotations
Step 2 - Download CDS/UTR sequence for annotations
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+**************************************************
Since we have found **SMN1**, this would be a convenient time to extract
the DNA sequence for the CDS and UTRs of the gene to use it as an
Since we have found **SMN1**, this would be a convenient time to extract
the DNA sequence for the CDS and UTRs of the gene to use it as an
Step 3 - Download gene and upstream/downstream sequence
Step 3 - Download gene and upstream/downstream sequence
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*******************************************************
Use the back button in your web browser to get back the **genome
browser view** of **SMN1** as shown below.
Use the back button in your web browser to get back the **genome
browser view** of **SMN1** as shown below.
Step 4 - Same/similar/related gene other species.
Step 4 - Same/similar/related gene other species.
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*************************************************
What good is a multiple sequence alignment viewer without multiple
sequences? Let'S find a similar gene in a few more species.
What good is a multiple sequence alignment viewer without multiple
sequences? Let'S find a similar gene in a few more species.
-~~~~~~~~~~~~~~~~~~~~~~~~
+************************
At this point you should have the annotations and fasta files for each
species. If you skipped the first four steps or are having trouble,
At this point you should have the annotations and fasta files for each
species. If you skipped the first four steps or are having trouble,